http://2008.igem.org/wiki/index.php?title=Team:Paris/Perspectives&feed=atom&action=historyTeam:Paris/Perspectives - Revision history2024-03-29T15:21:27ZRevision history for this page on the wikiMediaWiki 1.16.5http://2008.igem.org/wiki/index.php?title=Team:Paris/Perspectives&diff=105065&oldid=prevRomain.rousseau at 04:52, 30 October 20082008-10-30T04:52:20Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Paris/Menu}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Paris/Menu}}</div></td></tr>
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</table>Romain.rousseauhttp://2008.igem.org/wiki/index.php?title=Team:Paris/Perspectives&diff=104256&oldid=prevFelipe at 03:59, 30 October 20082008-10-30T03:59:45Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>One illustrative example is polyhydroxyalkanoic acids (PHAs), whose production/degradation cycle reduces undesirable wastes and emissions.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>One illustrative example is polyhydroxyalkanoic acids (PHAs), whose production/degradation cycle reduces undesirable wastes and emissions.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The biosynthesis of this polymer is currently subject to intensive work.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The biosynthesis of this polymer is currently subject to intensive work.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>It consists in expressing in appropriate quantities 3 enzymes PhaA ,PhaB and PhaC that <del class="diffchange diffchange-inline">sequentialy </del>process AcetoacetylcoA into its final product PHA.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>It consists in expressing in appropriate quantities 3 enzymes PhaA ,PhaB and PhaC that <ins class="diffchange diffchange-inline">sequentially </ins>process AcetoacetylcoA into its final product PHA.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This biosynthesis os subjected to 2 <del class="diffchange diffchange-inline">contraints </del>: </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This biosynthesis os subjected to 2 <ins class="diffchange diffchange-inline">constraints </ins>: </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Intermediate products of this pathway are used in alternatives competing metabolic pathways,</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Intermediate products of this pathway are used in alternatives competing metabolic pathways,</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Only the final product is of interest,so that all intermediates products need to be transformed into PHA.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Only the final product is of interest,so that all intermediates products need to be transformed into PHA.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:PHA.jpg|center]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:PHA.jpg|center]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Two strategies are commonly used in bioengineering of <del class="diffchange diffchange-inline">methabolics </del>pathways :</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Two strategies are commonly used in bioengineering of <ins class="diffchange diffchange-inline">metabolic </ins>pathways :</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Sequential expression of enzymes involved in the pathways,</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Sequential expression of enzymes involved in the pathways,</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Or constitutive expressions of all enzymes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Or constitutive expressions of all enzymes.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Because of the above mentioned limitations, none of these <del class="diffchange diffchange-inline">approches </del>are adapted here .</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Because of the above mentioned limitations, none of these <ins class="diffchange diffchange-inline">approaches </ins>are adapted here .</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Using the sequential expression, intermediate products would accumulate and thus be <del class="diffchange diffchange-inline">consummed </del>by competing pathways.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Using the sequential expression, intermediate products would accumulate and thus be <ins class="diffchange diffchange-inline">consumed </ins>by competing pathways.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Using the constitutive expression a mixture of final and intermediate product would necessarily be obtained.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Using the constitutive expression a mixture of final and intermediate product would necessarily be obtained.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our FIFO could be useful here. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our FIFO could be useful here. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Indeed a FIFO expression pattern is intermediate between a purely sequential and a purely constitutive expression.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Indeed a FIFO expression pattern is intermediate between a purely sequential and a purely constitutive expression.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>At some point all enzymes are presents ( no accumulation of intermediate products ) and during the last step only the last enzyme (PhC) is presents ( all intermediate products are <del class="diffchange diffchange-inline">consummed</del>).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>At some point all enzymes are presents ( no accumulation of intermediate products ) and during the last step only the last enzyme (PhC) is presents ( all intermediate products are <ins class="diffchange diffchange-inline">consumed</ins>).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Moreover, the fact that our system oscillate could provide to the cell a metabolic recovering phase.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Moreover, the fact that our system oscillate could provide to the cell a metabolic recovering phase.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== <del class="diffchange diffchange-inline">Bibliographie </del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== <ins class="diffchange diffchange-inline">Bibliography </ins>==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The type III secretion injectisome Guy R.Cornelis Nature reviews</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The type III secretion injectisome Guy R.Cornelis Nature reviews</div></td></tr>
</table>Felipehttp://2008.igem.org/wiki/index.php?title=Team:Paris/Perspectives&diff=104222&oldid=prevFelipe at 03:59, 30 October 20082008-10-30T03:59:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Example 1: type III secretion injectisome == </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Example 1: type III secretion injectisome == </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The <del class="diffchange diffchange-inline">Injectisome </del>is a complex nanomachine that allows bacteria to deliver protein effector across eucaryotic cellular membranes.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The <ins class="diffchange diffchange-inline">injectisome </ins>is a complex nanomachine that allows bacteria to deliver protein effector across eucaryotic cellular membranes.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The <del class="diffchange diffchange-inline">injectisom </del>consists of a basal structure, which resembles the basal structure of the flagellum, surmounted by either a needle, a needle and a filament or a long pilus.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The <ins class="diffchange diffchange-inline">injectisome </ins>consists of a basal structure, which resembles the basal structure of the flagellum, surmounted by either a needle, a needle and a filament or a long pilus.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:injectisome.JPG]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:injectisome.JPG]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The injectisome is related to the flagellum genetically. Studies show that a core of eight proteins share significant similarity with components of the flagellum. From this studies, we can expect that the genes expression is ruled by a sequential FIFO order so we can express it with our bacteriO'clock system .</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The injectisome is related to the flagellum genetically. Studies show that a core of eight proteins share significant similarity with components of the flagellum. From this studies, we can expect that the genes expression is ruled by a sequential FIFO order so we can express it with our bacteriO'clock system .</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Moreover, the injectisome will find a lot of applications for example ,bacteria that express this kinds of structure could :</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Moreover, the injectisome will find a lot of applications for example ,bacteria that express this kinds of structure could :</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* induce apoptosis of <del class="diffchange diffchange-inline">eucaryote </del>cells so we can developed by our system new kinds of in-vivo compatible anticancer therapy,</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* induce apoptosis of <ins class="diffchange diffchange-inline">Eukaryote </ins>cells so we can developed by our system new kinds of in-vivo compatible anticancer therapy,</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* be a new way to liberate substances produced by bacteria (see bioplastic application).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* be a new way to liberate substances produced by bacteria (see bioplastic application).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In conclusion, our <del class="diffchange diffchange-inline">bacterioclock </del>can be used in a lot of new applications like for instance, bottom-up molecular machines self-assembly or new kinds of optimized chemoreactor.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In conclusion, our <ins class="diffchange diffchange-inline">BacteriO'Clock </ins>can be used in a lot of new applications like for instance, bottom-up molecular machines self-assembly or new kinds of optimized chemoreactor.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Example 2: metabolic engineering of polyhydroxyalkanoate biosynthesis pathways ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Example 2: metabolic engineering of polyhydroxyalkanoate biosynthesis pathways ==</div></td></tr>
</table>Felipehttp://2008.igem.org/wiki/index.php?title=Team:Paris/Perspectives&diff=104132&oldid=prevPhilippe b at 03:56, 30 October 20082008-10-30T03:56:47Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The injectisome is related to the flagellum genetically. Studies show that a core of eight proteins share significant similarity with components of the flagellum. From this studies, we can expect that the genes expression is ruled by a sequential FIFO order so we can express it with our bacteriO'clock system .</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The injectisome is related to the flagellum genetically. Studies show that a core of eight proteins share significant similarity with components of the flagellum. From this studies, we can expect that the genes expression is ruled by a sequential FIFO order so we can express it with our bacteriO'clock system .</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Moreover, the injectisome will find a lot of applications for example ,bacteria that express this kinds of structure <del class="diffchange diffchange-inline">can </del>:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Moreover, the injectisome will find a lot of applications for example ,bacteria that express this kinds of structure <ins class="diffchange diffchange-inline">could </ins>:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* induce apoptosis of eucaryote cells so we can developed by our system new kinds of in-vivo compatible anticancer therapy,</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* induce apoptosis of eucaryote cells so we can developed by our system new kinds of in-vivo compatible anticancer therapy,</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* be a new way to liberate substances produced by bacteria (see bioplastic application).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* be a new way to liberate substances produced by bacteria (see bioplastic application).</div></td></tr>
</table>Philippe bhttp://2008.igem.org/wiki/index.php?title=Team:Paris/Perspectives&diff=104084&oldid=prevPhilippe b at 03:55, 30 October 20082008-10-30T03:55:47Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The injectisome is related to the flagellum genetically<del class="diffchange diffchange-inline">, studies </del>show that a core of eight proteins share significant similarity with components of the flagellum <del class="diffchange diffchange-inline"> from </del>this studies , we can <del class="diffchange diffchange-inline">expected </del>that the genes expression is <del class="diffchange diffchange-inline">rule </del>by a sequential FIFO order so we can express it with our <del class="diffchange diffchange-inline">bacterioclock </del>system .</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The injectisome is related to the flagellum genetically<ins class="diffchange diffchange-inline">. Studies </ins>show that a core of eight proteins share significant similarity with components of the flagellum<ins class="diffchange diffchange-inline">. From </ins>this studies, we can <ins class="diffchange diffchange-inline">expect </ins>that the genes expression is <ins class="diffchange diffchange-inline">ruled </ins>by a sequential FIFO order so we can express it with our <ins class="diffchange diffchange-inline">bacteriO'clock </ins>system .</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Moreover, the injectisome will find a lot of applications for example ,bacteria that express this kinds of structure can :</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Moreover, the injectisome will find a lot of applications for example ,bacteria that express this kinds of structure can :</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* induce apoptosis of eucaryote cells so we can developed by our system new kinds of in-vivo compatible anticancer therapy,</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* induce apoptosis of eucaryote cells so we can developed by our system new kinds of in-vivo compatible anticancer therapy,</div></td></tr>
</table>Philippe bhttp://2008.igem.org/wiki/index.php?title=Team:Paris/Perspectives&diff=104031&oldid=prevPhilippe b at 03:54, 30 October 20082008-10-30T03:54:40Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Injectisome is a complex nanomachine that allows bacteria to deliver protein effector across eucaryotic cellular membranes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Injectisome is a complex nanomachine that allows bacteria to deliver protein effector across eucaryotic cellular membranes.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The injectisom consists of a basal structure, which resembles the basal structure of the flagellum,surmounted by either a needle ,a needle and a filament <del class="diffchange diffchange-inline"> </del>or a long pilus.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The injectisom consists of a basal structure, which resembles the basal structure of the flagellum, surmounted by either a needle, a needle and a filament or a long pilus.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:injectisome.JPG]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:injectisome.JPG]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The injectisome is related to the flagellum genetically, studies show that a core of eight proteins share significant similarity with components of the flagellum from this studies , we can expected that the genes expression is rule by a sequential FIFO order so we can express it with our bacterioclock system .</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The injectisome is related to the flagellum genetically, studies show that a core of eight proteins share significant similarity with components of the flagellum from this studies , we can expected that the genes expression is rule by a sequential FIFO order so we can express it with our bacterioclock system .</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Moreover, the injectisome will find a lot of applications for example ,bacteria that express this kinds of structure can :</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Moreover, the injectisome will find a lot of applications for example ,bacteria that express this kinds of structure can :</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">Induce </del>apoptosis of eucaryote cells so we can developed by our system new kinds of in-vivo compatible anticancer therapy,</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">induce </ins>apoptosis of eucaryote cells so we can developed by our system new kinds of in-vivo compatible anticancer therapy,</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">Be </del>a new way to liberate <del class="diffchange diffchange-inline">bioplastic without killing our </del>bacteria (<del class="diffchange diffchange-inline">new kinds of optimized chemoreactor</del>).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">be </ins>a new way to liberate <ins class="diffchange diffchange-inline">substances produced by </ins>bacteria (<ins class="diffchange diffchange-inline">see bioplastic application</ins>).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In conclusion, our bacterioclock can be used in a lot of new applications like for instance, bottom-up molecular machines self-assembly or new kinds of optimized chemoreactor.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In conclusion, our bacterioclock can be used in a lot of new applications like for instance, bottom-up molecular machines self-assembly or new kinds of optimized chemoreactor.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:PHA.jpg|center]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:PHA.jpg|center]]</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">two </del>strategies are commonly used in bioengineering of methabolics pathways :</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Two </ins>strategies are commonly used in bioengineering of methabolics pathways :</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Sequential expression of enzymes involved in the pathways,</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Sequential expression of enzymes involved in the pathways,</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Or constitutive expressions of all enzymes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Or constitutive expressions of all enzymes.</div></td></tr>
</table>Philippe bhttp://2008.igem.org/wiki/index.php?title=Team:Paris/Perspectives&diff=103936&oldid=prevPhilippe b at 03:52, 30 October 20082008-10-30T03:52:22Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As for the flagella biosynthesis, our FIFO could be useful for many bottom-up assembled molecular machines that needs to be assembled in a precise order.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As for the flagella biosynthesis, our FIFO could be useful for many <ins class="diffchange diffchange-inline">'''</ins>bottom-up assembled molecular machines<ins class="diffchange diffchange-inline">''' </ins>that needs to be assembled in a precise order.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Moreover, as we will show on an example, the FIFO could also be useful in many biosynthetic pathways subject to competitive alternative pathways and for wich intermediate products must be avoided.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Moreover, as we will show on an example, the FIFO could also be useful in many <ins class="diffchange diffchange-inline">'''</ins>biosynthetic pathways<ins class="diffchange diffchange-inline">''' </ins>subject to competitive alternative pathways and for wich intermediate products must be avoided.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We will develop below two examples: type III secretion injectisome and PHA biosynthesis pathway.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We will develop below two examples: type III secretion injectisome and PHA biosynthesis pathway.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Example 1: type III secretion injectisome == </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Example 1: type III secretion injectisome == </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">As for the flagella biosynthesis, our FIFO could be useful for many bottom-up assembled molecular machines that needs to be assembled in a precise order.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">For instance ,the type III secretion injectisome.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Injectisome is a complex nanomachine that allows bacteria to deliver protein effector across eucaryotic cellular membranes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Injectisome is a complex nanomachine that allows bacteria to deliver protein effector across eucaryotic cellular membranes.</div></td></tr>
</table>Philippe bhttp://2008.igem.org/wiki/index.php?title=Team:Paris/Perspectives&diff=103900&oldid=prevPhilippe b at 03:51, 30 October 20082008-10-30T03:51:34Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As for the flagella biosynthesis, our FIFO could be useful for many bottom-up assembled molecular machines that needs to be assembled in a precise order.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As for the flagella biosynthesis, our FIFO could be useful for many bottom-up assembled molecular machines that needs to be assembled in a precise order.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">For instance </del>,the type III secretion injectisome.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Moreover, as we will show on an example</ins>, the <ins class="diffchange diffchange-inline">FIFO could also be useful in many biosynthetic pathways subject to competitive alternative pathways and for wich intermediate products must be avoided.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We will develop below two examples: </ins>type III secretion injectisome <ins class="diffchange diffchange-inline">and PHA biosynthesis pathway</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== <del class="diffchange diffchange-inline">Complexes nanomachine Factory </del>== </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== <ins class="diffchange diffchange-inline">Example 1: type III secretion injectisome </ins>== </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As for the flagella biosynthesis, our FIFO could be useful for many bottom-up assembled molecular machines that needs to be assembled in a precise order.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As for the flagella biosynthesis, our FIFO could be useful for many bottom-up assembled molecular machines that needs to be assembled in a precise order.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For instance ,the type III secretion injectisome.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For instance ,the type III secretion injectisome.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In conclusion, our bacterioclock can be used in a lot of new applications like for instance, bottom-up molecular machines self-assembly or new kinds of optimized chemoreactor.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In conclusion, our bacterioclock can be used in a lot of new applications like for instance, bottom-up molecular machines self-assembly or new kinds of optimized chemoreactor.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== Example <del class="diffchange diffchange-inline">of an application</del>: <del class="diffchange diffchange-inline">Metabolic </del>engineering of polyhydroxyalkanoate biosynthesis pathways ==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== Example <ins class="diffchange diffchange-inline">2</ins>: <ins class="diffchange diffchange-inline">metabolic </ins>engineering of polyhydroxyalkanoate biosynthesis pathways ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Human overpopulation combined with the current lifestyle urges the rational, efficient, and sustainable use of natural resources to produce environmentally friendly plastic materials.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Human overpopulation combined with the current lifestyle urges the rational, efficient, and sustainable use of natural resources to produce environmentally friendly plastic materials.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>One illustrative example is polyhydroxyalkanoic acids (PHAs), whose production/degradation cycle reduces undesirable wastes and emissions.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>One illustrative example is polyhydroxyalkanoic acids (PHAs), whose production/degradation cycle reduces undesirable wastes and emissions.</div></td></tr>
</table>Philippe bhttp://2008.igem.org/wiki/index.php?title=Team:Paris/Perspectives&diff=103781&oldid=prevPhilippe b: /* Complexes nanomachine Factory */2008-10-30T03:47:14Z<p><span class="autocomment">Complexes nanomachine Factory</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Paris/Menu}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Paris/Menu}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">As for the flagella biosynthesis, our FIFO could be useful for many bottom-up assembled molecular machines that needs to be assembled in a precise order.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">For instance ,the type III secretion injectisome.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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</table>Philippe bhttp://2008.igem.org/wiki/index.php?title=Team:Paris/Perspectives&diff=103761&oldid=prevPhilippe b: /* Bibliographie */2008-10-30T03:46:40Z<p><span class="autocomment">Bibliographie</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Bibliographie ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Bibliographie ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">"</del>The type III secretion injectisome <del class="diffchange diffchange-inline">" </del>Guy R.Cornelis Nature reviews</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* </ins>The type III secretion injectisome <ins class="diffchange diffchange-inline"> </ins>Guy R.Cornelis Nature reviews</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Process design for microbial plastic factories: metabolic engineering of polyhydroxyalkanoates </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Ilana S Aldor and Jay D Keasling</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Department of Chemical Engineering, 201 Gilman Hall, University of California, Berkeley, 94720-1462, USA </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">23 September 2003</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* PHA synthase engineering toward superbiocatalysts for custom-made biopolymers.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Nomura CT, Taguchi S.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Department of Chemistry, State University of New York - College of Environmental Science and Forestry, 121 Jahn Laboratory, Syracuse, NY 13210, USA.</ins></div></td></tr>
</table>Philippe b