Team:PennState

From 2008.igem.org

(Difference between revisions)
 
(37 intermediate revisions not shown)
Line 1: Line 1:
<html>
<html>
-
<!--
 
-
  Landing page
 
-
  Contains links to all other pages of the site as well as a quick introduction to the projects
 
-
  The aim of this page was to be visually appealing and contain a very small amount of text so that the user would be given a quick introduction to the project. The  user has only to read several lines to get a good grasp of what we did. This will encourage them to explore deeper.
 
-
-->
 
<head>
<head>
-
<!--
 
-
  Cascading Style Sheet (CSS) component
 
-
  in plain html style sheets are completely independent of the HTML page but are included in this page to simplify design within a wiki script environment.
 
-
  This section describes the layout, colors and images displayed on the page
 
-
-->
 
<style>
<style>
-
 
#globalWrapper {
#globalWrapper {
  background-image: url("https://static.igem.org/mediawiki/2008/e/e4/Bglogo.png") !important;
  background-image: url("https://static.igem.org/mediawiki/2008/e/e4/Bglogo.png") !important;
  background-repeat: repeat;
  background-repeat: repeat;
 +
}
}
Line 125: Line 115:
#pagecontent p {
#pagecontent p {
  text-indent: 1.25em;
  text-indent: 1.25em;
 +
}
 +
 +
p.start:first-line {
 +
font-weight: bold;
 +
letter-spacing: 1.2;
}
}
</style>
</style>
Line 134: Line 129:
<!-- First table displays centered iGEM 2007 Logo -->   
<!-- First table displays centered iGEM 2007 Logo -->   
<div id="header">
<div id="header">
-
  <img src="https://static.igem.org/mediawiki/2008/d/df/Penn_state_igem_logo.JPG" alt="Penn State" />
+
  <img src="https://static.igem.org/mediawiki/2008/a/ad/Penn_state_igem_logo2.JPG" alt="Penn State" />
</div>
</div>
Line 156: Line 151:
-->
-->
<td valign="top" width="20%" id="projectnav">
<td valign="top" width="20%" id="projectnav">
-
  <h4 style="margin-top: 0;">Hormone Biosensors</h4>
+
  <h4>Diauxie Elimination</h4>
  <dl id="hbnav">
  <dl id="hbnav">
-
   <dd><a href="hbintro" title="Intro to Endocrine Disruption, pseudoestrogens, pthalates, nuclear hormone receptors, and our goals">Introduction</a></dd>
+
   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/intro">Introduction</a></dd>
-
  <dt>Smart Fold</dt>
+
   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/TheSystem">The System</a></dd>
-
   <dd><a href="https://2008.igem.org/Team:PennState/smartfold/overview">Overview</a></dd>
+
   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/Strategies">Strategies</a></dd>
-
   <dd><a href="https://2008.igem.org/Team:PennState/smartfold/parts" title="Parts submitted to the registry for this project">Parts</a></dd>
+
   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/progress">Progress</a></dd>
-
   <dd><a href="https://2008.igem.org/Team:PennState/smartfold/references">References</a></dd>
+
   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/conclusions">Conclusions</a></dd>
-
  <dt>Nuclear Fusion</dt>
+
   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/parts" title="Parts submitted to the registry for diauxie">Parts</a></dd>
-
   <dd><a href="https://2008.igem.org/Team:PennState/fusion/overview">Overview</a></dd>
+
   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/references">References</a></dd>
-
   <dd><a href="https://2008.igem.org/Team:PennState/fusion/parts" title="Parts submitted to the registry for this project">Parts</a></dd>
+
-
   <dd><a href="https://2008.igem.org/Team:PennState/fusion/references">References</a></dd>
+
  </dl>
  </dl>
-
  <h4>Diauxie Elimination</h4>
+
  <h4><acronym title="Nuclear Hormone Receptor">NHR Biosensors</acronym><br/></h4>
  <dl id="denav">
  <dl id="denav">
-
   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/intro">Introduction</a></dd>
+
   <dd><a href="https://2008.igem.org/Team:PennState/NHR/introduction">NHR Introduction</a></dd>
-
   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/overview">Overview</a></dd>
+
 
-
   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/parts" title="Parts submitted to the registry for this project">Parts</a></dd>
+
   <dd><a href="https://2008.igem.org/Team:PennState/smartfold/overview">Phthalate Biosensor</a></dd>
-
  <dd><a href="https://2008.igem.org/Team:PennState/diauxie/references">References</dd>
+
 
 +
   <dd><a href="https://2008.igem.org/Team:PennState/fusion/overview">BPA Biosensor</a></dd>
  </dl>
  </dl>
</td>
</td>
<!-- Main content area -->
<!-- Main content area -->
-
<td valign="top" id="pagecontent"><span style="font-size: 16pt">PENN STATE iGEM 2008</span>
+
<td valign="top" id="pagecontent" width="80%"><span style="font-size: 16pt">Welcome to Penn State iGEM 2008</span>
  <hr />
  <hr />
-
   <p>Welcome to the Penn State iGEM 2008 team’s website. We are currently working hard at a few different projects for this year's competition. Our main focus for 2008 was the elimination of diauxie in <em>E. coli</em> to create a xylose inducible system independent of glucose regulation. This system could be used for creating more efficient bioproduction by altering the utilization of 5 and 6 carbon sugars.</p>
+
   <p class="start"><a href="http://www.psu.edu" title="Pennsylvania State University" target="_blank">Penn State University</a> has participated in the International Genetically Engineered Machines (iGEM) competition for four years as of 2008, and we are excited to participate once more. Our team consists of 5 undergraduate students, one visiting undergrad and one high school student who work independently, coordinated through weekly meetings with our advisers. Check out our <a href="https://2008.igem.org/Team:PennState/Team" title="Penn State's 2008 iGEM team">Team Page</a> to meet us!</p>
-
   <p>We also have been working on creating biosensors that use human nuclear hormone receptors to recognize potentially harmful compounds. These receptor systems occur naturally in the human body, but our goal is to retain and utilize their functions in <em>E. coli</em>. Please explore our website to find out more about us and our projects!</p>
+
<p><strong>Our main focus for 2008 was the elimination of <a href="https://2008.igem.org/Team:PennState/diauxie/intro">diauxie</a> in <em>E. coli</em> to create a xylose inducible system independent of glucose regulation.</strong> This system could be used for creating more efficient bioproduction by altering the utilization of 5 and 6 carbon sugars. Please check out the links on the left to navigate through our work.</p>
-
  <p> If there are any questions or comments about the information on this site please contact us at <a href="mailto:gjt5001@psu.edu" title="email us">gjt5001@psu.edu</a>. </p>
+
   <p>Two other projects begun this year focus on creating <a href="https://2008.igem.org/Team:PennState/NHR/introduction"What kind of 'Biosensors' are we talking about?">biosensors</a> that use human nuclear hormone receptors to recognize potentially harmful compounds. These receptor systems occur naturally in the human body, but our goal is to retain and utilize their functions in <em>E. coli</em>. The links on the left should introduce you to our thought process and progress on each of these projects, and provide a fuller introduction to the topic.</p>
-
+
  <p> Please explore our website to find out more about us and our projects! If there are any questions or comments about the information on this site please feel free to contact us at <a href="mailto:gjt5001@psu.edu" title="email us">gjt5001@psu.edu</a>. </p>
-
<tr>
+
 
-
    <td colspan="2" style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size: 14pt">Diauxie Elimination by Xylose Inducible Promoters </span>
+
    <span style="font-size: 14pt"><a href="https://2008.igem.org/Team:PennState/MedalChecklist">Medal Checklist</a></span>
-
      <hr />
+
 
-
      <p><img src="picture here" alt="[img]" style="float:left; margin:5px;"/>Microorganisms typically preferentially utilize glucose over other sugar carbon sources such as xylose. This is largely regulated through control of gene expression based on the response of regulatory elements to sugars available to the cell. In <em>E. coli</em>, the xylose metabolism operon is controlled by both the xylose-inducible XylR activator protein and the cAMP receptor protein (CRP). In this project we attempt to eliminate glucose control over xylose-inducible gene expression in <em>E. coli</em> by altering the natural transcriptional control region of the xylose operon. Designs constructed and tested include scrambling the CRP binding site, increasing the strength of the xyl promoter, and overexpressing XylR. Xylose-inducible gene expression that functions independently of glucose regulation provides a useful approach to improving microbial utilization of biomass feedstocks containing mixtures of glucose and xylose.</p>
+
<table>
 +
  <tr>
 +
  <td style="padding-top:30px; padding-right:30px" valign="top" width="45%">
 +
    <h3>Related Links</h3>
 +
    <hr />
 +
    <ul>
 +
      <li><a href="http://partsregistry.org/Main_Page" title="Parts Registry">Parts Registry</a></li>
 +
 
 +
<li><a href="http://www.che.psu.edu/Faculty/Cirino/" title="Prof. Pat Cirino's Lab website" target="_blank">Prof. Pat Cirino's Lab website</a></li>
 +
<li><a href="http://www.abe.psu.edu/fac/Richard/Overview.htm" title="Prof. Tom Richard's Lab website" target="_blank">Prof. Tom Richard's Lab website</a></li>
 +
<li><a href="http://openwetware.org/wiki/IGEM:PennState" title="Penn State iGEM OpenWetWare" target="_blank">Penn State iGEM OpenWetWare</a></li>
 +
 
 +
 
 +
      <li><a href="http://www.princeton.edu/che/people/faculty/wood/" title="David Wood" target="_blank">David Wood</a></li>
 +
      <li><a href="http://www.cmtc.psu.edu/" title="Center for Molecular Toxicology and Carcinogenesis" target="_blank">PennState Center for Molecular Toxicology and Carcinogenesis</a></li>
 +
    </ul>
   </td>
   </td>
-
  </tr>  
+
  <td style="padding-top:30px; padding-right:30px" valign="top" width="45%">
 +
    <h3>Drew Endy On Synthetic Biology</h3>
 +
      <hr />
 +
<!--
 +
  This division block contains the code to import and display a flash object
 +
  Specifically importing and formatting a video uploaded onto "youtube"
 +
-->
 +
            <div style="margin:10px">
-
<table style="padding-left: 0"> <!-- change padding to re-indent this content segment -->
+
<object width="205" height="200">
-
        <tr><td style="padding-top:30px; padding-right:30px" valign="top" width="90%"><span style="font-size:14pt">Hormone Prescreening <em>E. coli</em></span>
+
<param name="movie" value="http://www.youtube.com/v/XIuh7KDRzLk&hl=en&fs=1"></param><param name="allowFullScreen" value="true">
-
      <hr />
+
</param>
-
      <p>Two of our projects aim to construct biosensors which will ultimately serve as a water prescreening tool.  The focus of these biosensors will be to detect phthalate compounds utilizing the Peroxisome Proliferator Activated Receptor (PPAR) and detecting Bisphenol A (BPA) by the Estrogen Receptor (ER).  Recent studies show phthalates cause negative health effects such as damage to the liver and kidneys and birth defects in rodents.  Phthalates are introduced into our environment by their use as plastisizers for materials ranging from polyvinyl chloride to nail polish to small toys.  BPA is also found in plastics but instead it is used for the synthesis of hard plastics.  Once BPA enters the human body it is confused for estrogen and parallels the effects of estrogen after attaching to the ligand binding region of the ER.  Analytical detection methods for water contamination are compound specific and very costly.  Having a simple and cheap tool to screen for phthalates or BPA as a general class of compounds would eliminate the cost and time involved in detection.</p>
+
<embed src="http://www.youtube.com/v/XIuh7KDRzLk&hl=en&fs=1" type="application/x-shockwave-flash" allowfullscreen="true" width="205" height="200">
-
      <p> We are using two of the natural human nuclear hormone receptor proteins that recognize a large class of ligands, and attempting to express them heterologously in <em>E. Coli</em>.  The complexity of this mammalian protein makes it difficult to express it in a prokaryote.  We have two different strategies to express and use these receptors to detect compounds in <em>E. Coli</em>.  </p>
+
</embed>
 +
</object>
 +
            </div>
 +
    </td>
-
      <table> <!-- this table separates content into column-like quadrants -->
 
-
        <tr>
 
-
          <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size: 14pt">Smart Fold Reporter</span>
 
-
            <hr />
 
-
            <img src="http://upload.wikimedia.org/wikipedia/en/8/87/PPARg.png" alt="[img]" style="float:left; margin:5px;width: 30%"/>
 
-
            <p>This <em>Smart Fold Reporter</em> project uses altered growth conditions so that the entire <acronym title="Human Peroxisome Proliferator Activated Receptor subtype Alpha">hPPARα</acronym> protein is successfully expressed in <em>E. coli</em> and used to transcriptionally report for the presence of phthalates. Rather than changing the receptor and possibly losing its usefulness, we are chemically optimizing the cell environment. Check out our Overview to find out how.
 
-
</p></td>
 
-
          <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size:18px">Nuclear Fusion</span>
 
-
            <hr />
 
-
<img src="https://static.igem.org/mediawiki/2008/d/d9/PSU2008iGEM_BPAimage.png" alt="[img]" style="float:left; margin:5px;width: 30%;"/>
+
 
-
            <p> The <em>Nuclear Fusion</em> project involves a plasmid construct very generously donated to our iGEM team from David W. Wood, Department of Chemical Engineering at Princeton University.  Research in their lab has constructed a biosensor containing just the ligand binding domain (LBD) of the estrogen receptor (ER). Our plan for this project is to work on the sensitivity of the biosensor in hopes of using this for water prescreens, similar to the <em>Smart Fold Reporter</em> project.  The sensitivity will be focused for BPA which has a very different conformation than the natural agonist of the ER system (estradiol).  This difference causes BPA to bind weakly but still disturbs normal ER function.</p><p>We intend to analyze the LBD of ER and perform directed evolution to increase BPA sensitivity.  During directed evolution, certain regions of the ER LBD would be targeted for random mutagenesis providing a library of mutants in the trillions.  The mutant library would be induced with BPA and the best growing colony would be selected, tested, and mutated for further sensitivity.</p>
+
<td style="padding-top:30px; padding-right:30px" valign="top" width="45%">
-
</p></td>
+
    <h3>Garrett Tobin On Diauxie Elimination</h3>
-
        </tr>
+
      <hr />
 +
<!--
 +
  This division block contains the code to import and display a flash object
 +
  Specifically importing and formatting a video uploaded onto "youtube"
 +
-->
-
  <tr>
+
 
-
  <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size: 14pt">Quick Links</span>
+
<div style="margin:10px">
-
            <hr />
+
    <object width="205" height="200">
-
            <br/>
+
      <param name="movie" value="http://www.youtube.com/v/QA-tETsb3Wo&hl=en&fs=1">
-
            <p><a href="http://parts.mit.edu/igem07/index.php/PennState/final_result#contributions" title="Our Contribution To the Registry">Table of our Contributions to the Registry</a></p>
+
      </param>
-
            <p><a href="http://parts.mit.edu/igem07/images/7/73/IgemDesign.swf" title="Interactive Schematic" target="_blank">Interactive <em>E. co</em>Lisa Schematic</a></p>
+
      <param name="allowFullScreen" value="true">
-
            <p> <a href="http://www.psu.edu" title="Pennsylvania State University" target="_blank">Pennsylvania State University</a> </p>
+
      </param>
-
            <p> <a href="http://www.environment.psu.edu/" title="Penn State Institutes of Energy and the Environment" target="_blank">Penn State Institutes of Energy and the Environment</a> </p>
+
      <embed src="http://www.youtube.com/v/QA-tETsb3Wo&hl=en&fs=1" type="application/x-shockwave-flash" allowfullscreen="true" width="205" height="200">
-
    <p> <a href="http://www.cmtc.psu.edu/" title="Center for Molecular Toxicology and Carcinogenesis" target="_blank">Center for Molecular Toxicology and Carcinogenesis</a> </p>
+
      </embed>
 +
    </object>
             </div>
             </div>
-
          </td>
+
    </td>
-
          <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size: 14pt">Drew Endy On Synthetic Biology</span>
+
</tr>
-
            <hr />
+
</table>
-
+
 
-
<!--
+
-
    This division block contains the code to import and display a flash object
+
-
Specifically importing and formatting a video uploaded onto "youtube"
+
-
-->
+
-
            <div style="margin:10px">
+
-
              <object width="200">
+
-
                <param name="movie" value="http://www.youtube.com/v/XIuh7KDRzLk&rel=1">
+
-
                </param>
+
-
                <param name="wmode" value="transparent">
+
-
                </param>
+
-
                <embed src="http://www.youtube.com/v/XIuh7KDRzLk&rel=1" type="application/x-shockwave-flash" wmode="transparent" width="200" ></embed>
+
-
              </object>
+
-
            </div></td>
+
-
        </tr>
+
-
      </table>
+
</td></tr></table> <!-- end the table for content quadrants -->
</td></tr></table> <!-- end the table for content quadrants -->
</td></tr></table> <!-- end content block that is adjacent to sidelinks -->
</td></tr></table> <!-- end content block that is adjacent to sidelinks -->

Latest revision as of 03:25, 30 October 2008

Home The Team The Project Parts Notebook

Diauxie Elimination

Introduction
The System
Strategies
Progress
Conclusions
Parts
References

NHR Biosensors

NHR Introduction
Phthalate Biosensor
BPA Biosensor
 Welcome to Penn State iGEM 2008

Penn State University has participated in the International Genetically Engineered Machines (iGEM) competition for four years as of 2008, and we are excited to participate once more. Our team consists of 5 undergraduate students, one visiting undergrad and one high school student who work independently, coordinated through weekly meetings with our advisers. Check out our Team Page to meet us!

Our main focus for 2008 was the elimination of diauxie in E. coli to create a xylose inducible system independent of glucose regulation. This system could be used for creating more efficient bioproduction by altering the utilization of 5 and 6 carbon sugars. Please check out the links on the left to navigate through our work.

Two other projects begun this year focus on creating biosensors that use human nuclear hormone receptors to recognize potentially harmful compounds. These receptor systems occur naturally in the human body, but our goal is to retain and utilize their functions in E. coli. The links on the left should introduce you to our thought process and progress on each of these projects, and provide a fuller introduction to the topic.

Please explore our website to find out more about us and our projects! If there are any questions or comments about the information on this site please feel free to contact us at gjt5001@psu.edu.

Medal Checklist

Related Links

Drew Endy On Synthetic Biology

Garrett Tobin On Diauxie Elimination

Sponsors for our team! Thanks so much!

invitrogen Dupont

Retrieved from "http://2008.igem.org/Team:PennState"