Constructs
Saccharomyces cerevisiae are widely used for baking and brewing, and they are particular useful for synthesizing metabolites under fermentation conditions which prevent the air oxidation of many useful compounds. To achieve our project and expand the synthetic biology toolbox for programming yeast, we have introduced into the iGem registry BioBricks encoding 3 yeast promoters, 3 yeast terminators, a two micron origin of replication, 2 selectable markers, 2 enzymes, and a yeast integration plasmid. In addition, we have generated seven constructs using these parts. Furthermore, we have submitted two additional parts representing a foundational tool, including a gene encoding an amber suppressed RFP biobrick for screening of SupF+ (Amber suppressor) genotype and an amber suppressor tRNA biobrick.
Yeast Promoters
BBa_K122000 |
pPGK1 |
This is the 1500 bp upstream of the PGK1 coding region in an industrial yeast strain. Constitutive promoter. |
1497 |
BBa_K122002 |
pADH1 |
700bp upstream of ADH1 promoter region containing RBS. Constitutive promoter. |
701 |
BBa_K122017 |
pGAL1 + tetO |
The GAL1 promoter which is highly repressed by glucose. An additional tetracycline operator site was included upstream of the RBS to allow repression by tetR. |
484 |
Yeast Terminators
BBa_K122003 |
tCYC1 |
300bp downstream the CYC1 coding region in a standard yeast strain. |
300 |
BBa_K122004 |
tADH1 |
300bp downstream the ADH1 coding region in a standard yeast strain. |
300 |
BBa_K122013 |
tPGK1 |
1000bp downstream the PGK1 coding region in an industrial yeast strain. |
1000 |
Selectable Markers
BBa_K122018 |
ZeoR |
Zeocin Resistance Gene |
300 |
BBa_K122008 |
BleoR |
Bleocin Resistance Gene under pTet promoter |
800ish |
BBa_K122014 |
ORI+HisTag |
2 Micron ORI and Histadine Tag |
<9000 |
Project Specific Constructs
Additional Bacterial Parts
Novel Zero Leak Inverter Concept
wtRFP in SupF- / ARFP in SupF- /wtRFP in SupF+ / ARFP in SupF+
wtRFP in SupF- / ARFP in SupF- /wtRFP in SupF+ / ARFP in SupF+
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