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Team:Rice University/Notebook/20 June 2008
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Friday, 20 June
- Taylor Stevenson:
- VCS257 SupA+ cells were grown O/N @ 37*C in Lambda media. 4.5mL culture was infected with 4.5uL of phage packaging product from November, 2007 and allowed to incubate for 20min @ 37*C shaking @ 250 rpm. Infected culture was heat shocked @ 42*C for 20min, inducing lysis by denaturing the CI857 repressor. Cells were then grown @ 37*C shaking @ 250 rpm, and their optical density was measured for XXXXX hours.
