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Team:The University of Alberta/12 June 2008
From 2008.igem.org
Prefix/suffix TetR Binding Site/Promoter GENE and HIS tag and Double Stop
BisdA
gaattcgcggccgcttctagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacatgcctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaataccggtcacggccgaactggatggccttaagctcacgatcccgccggactcccatcaccatcaccatcactgatgatactagtagcggccgctgcag
BisdB (I had to change one base pair to get rid of an EcoRI site)
gaattcgcggccgcttctagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacatgaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaactgacatcaccatcaccatcactgatgatactagtagcggccgctgcag
