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Team:The University of Alberta/17 June 2008
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==Troubleshooting Results== | ==Troubleshooting Results== | ||
'''Saima'''<br> | '''Saima'''<br> | ||
| - | Tried doing digests from Jason's digests of the genes in pUC57 (see below) using columns from three different kits. The results were all similar - very low concentrations for each. Next we are going to try running already purified DNA of a known, high concentration through the columns and measure the concentration that is eluted to determine how much, if any, is sticking to the column | + | Tried doing digests from Jason's digests of the genes in pUC57 (see below) using columns from three different kits. The results were all similar - very low concentrations for each. Next we are going to try running already purified DNA of a known, high concentration through the columns and measure the concentration that is eluted to determine how much, if any, is sticking to the column<br> |
'''Jason'''<br> | '''Jason'''<br> | ||
Is making a single cut with Xba in j61003 plasmid to attempt to ligate it back together and thereby testing our ligation buffer and enzyme | Is making a single cut with Xba in j61003 plasmid to attempt to ligate it back together and thereby testing our ligation buffer and enzyme | ||
Jason is also redoing the Purple Russian J61003 ligation using new ligation buffer | Jason is also redoing the Purple Russian J61003 ligation using new ligation buffer | ||
Revision as of 19:13, 17 June 2008
Today
Today we are continuing the troubleshooting from yesterday.
Troubleshooting Results
Saima
Tried doing digests from Jason's digests of the genes in pUC57 (see below) using columns from three different kits. The results were all similar - very low concentrations for each. Next we are going to try running already purified DNA of a known, high concentration through the columns and measure the concentration that is eluted to determine how much, if any, is sticking to the column
Jason
Is making a single cut with Xba in j61003 plasmid to attempt to ligate it back together and thereby testing our ligation buffer and enzyme
Jason is also redoing the Purple Russian J61003 ligation using new ligation buffer
