Team:The University of Alberta/23 July 2008

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Revision as of 17:40, 23 July 2008 by Gard 6 (Talk | contribs)
  • Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI); 2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV (digest was treated with Antartic Phosphatase); 3) conc. digest of J61003 with (SpeI+PstI) (digest was treated with Antartic Phosphatase).
  • Minipreped TetR O/N's set up on Tuesday night. It is in MininPrep Box 3.
  • Digested B0034 with Spe/Pst, and TetR with XbaI/Pst. Digested the biobrick Lac1 ERE with Spe/Pst. Running them on 1% gel.
  • Digested TetR with Xba/Pst
  • Digested LacIQERE with Spe/Pst
  • Degested B0034 with spe/pst
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