Team:The University of Alberta/24 July 2008

From 2008.igem.org

(Difference between revisions)
(New page: ==System 2== Today we have taken the ligation from yesterday and transformed it as well as digested it. *if all goes well the digested product should be B0034+TETR *we will then ligate th...)
Line 3: Line 3:
*if all goes well the digested product should be B0034+TETR
*if all goes well the digested product should be B0034+TETR
*we will then ligate the B0034+TETR with LacIQERE giving us a complete system 2.
*we will then ligate the B0034+TETR with LacIQERE giving us a complete system 2.
 +
*Digested pTet with Spe/Pst.
 +
*Digested RFP with Xba/Pst.

Revision as of 16:21, 24 July 2008

System 2

Today we have taken the ligation from yesterday and transformed it as well as digested it.

  • if all goes well the digested product should be B0034+TETR
  • we will then ligate the B0034+TETR with LacIQERE giving us a complete system 2.
  • Digested pTet with Spe/Pst.
  • Digested RFP with Xba/Pst.