Team:The University of Alberta/24 October 2008

From 2008.igem.org

(Difference between revisions)
(David)
(David)
Line 8: Line 8:
i gel purified the desired products.
i gel purified the desired products.
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i set up ligations for pSB103+laqIQ-ERE-TetR-pTET-RFP, pSB103+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C.
+
i set up ligations for pSB1A3+laqIQ-ERE-TetR-pTET-RFP, pSB1A3+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C.

Revision as of 03:21, 29 October 2008

David

i digested

          laqIQ-ERE-TetR-pTET-RFP with XbaI+PstI,
          pTET-BisdA-pTET-BisdB with XbaI+PstI,
          pUC57-laqIQ-ERE-TetR-pTET-RFP with SphI+PstI and treated with CIP.

i gel purified the desired products.

i set up ligations for pSB1A3+laqIQ-ERE-TetR-pTET-RFP, pSB1A3+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C.