Team:The University of Alberta/24 October 2008

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David

i digested

          laqIQ-ERE-TetR-pTET-RFP with XbaI+PstI,
          pTET-BisdA-pTET-BisdB with XbaI+PstI,
          pUC57-laqIQ-ERE-TetR-pTET-RFP with SphI+PstI and treated with CIP.

i gel purified the desired products.

i set up ligations for pSB103+laqIQ-ERE-TetR-pTET-RFP, pSB103+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C.