Team:The University of Alberta/28 October 2008

From 2008.igem.org

(Difference between revisions)
(New page: ==David== today i did a pcr check of candidate colonies for pSB103-ER, pSB103-laqIQ-ERE-TetR-pTET-RFP, pSB103-pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP-pTET-BisdA-pTET-BisdB. ...)
(David)
 
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==David==  
==David==  
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today i did a pcr check of candidate colonies for pSB103-ER, pSB103-laqIQ-ERE-TetR-pTET-RFP, pSB103-pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP-pTET-BisdA-pTET-BisdB.
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today i did a pcr check of candidate colonies for  
 +
  pSB1A3-ER
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  pSB1A3-laqIQ-ERE-TetR-pTET-RFP
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  pSB1A3-pTET-BisdA-pTET-BisdB
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  pUC57-laqIQ-ERE-TetR-pTET-RFP-pTET-BisdA-pTET-BisdB.
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pSB103-ER  worked
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  pSB1A3-ER  worked
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pSB103-laqIQ-ERE-TetR-pTET-RFP did NOT work
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  pSB1A3-laqIQ-ERE-TetR-pTET-RFP did NOT work
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pSB103-pTET-BisdA-pTET-BisdB worked
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  pSB1A3-pTET-BisdA-pTET-BisdB worked
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pUC57-laqIQ-ERE-TetR-pTET-RFP-pTET-BisdA-pTET-BisdB worked  
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  pUC57-laqIQ-ERE-TetR-pTET-RFP-pTET-BisdA-pTET-BisdB worked  
today we also aliquoted all of our vectors to shipping off to iGEM central.  goodbye sweet vectors.  
today we also aliquoted all of our vectors to shipping off to iGEM central.  goodbye sweet vectors.  
i have not yet examined yesterdays sequences.
i have not yet examined yesterdays sequences.

Latest revision as of 03:24, 29 October 2008

David

today i did a pcr check of candidate colonies for

 pSB1A3-ER
 pSB1A3-laqIQ-ERE-TetR-pTET-RFP
 pSB1A3-pTET-BisdA-pTET-BisdB
 pUC57-laqIQ-ERE-TetR-pTET-RFP-pTET-BisdA-pTET-BisdB.
 pSB1A3-ER  worked
 pSB1A3-laqIQ-ERE-TetR-pTET-RFP did NOT work
 pSB1A3-pTET-BisdA-pTET-BisdB worked
 pUC57-laqIQ-ERE-TetR-pTET-RFP-pTET-BisdA-pTET-BisdB worked 

today we also aliquoted all of our vectors to shipping off to iGEM central. goodbye sweet vectors.

i have not yet examined yesterdays sequences.