Team:The University of Alberta/28 October 2008

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David

today i did a pcr check of candidate colonies for

 pSB1A3-ER
 pSB1A3-laqIQ-ERE-TetR-pTET-RFP
 pSB1A3-pTET-BisdA-pTET-BisdB
 pUC57-laqIQ-ERE-TetR-pTET-RFP-pTET-BisdA-pTET-BisdB.

 pSB1A3-ER  worked
 pSB1A3-laqIQ-ERE-TetR-pTET-RFP did NOT work
 pSB1A3-pTET-BisdA-pTET-BisdB worked
 pUC57-laqIQ-ERE-TetR-pTET-RFP-pTET-BisdA-pTET-BisdB worked

today we also aliquoted all of our vectors to shipping off to iGEM central. goodbye sweet vectors.

i have not yet examined yesterdays sequences.