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Team:The University of Alberta/2 June 2008
From 2008.igem.org
(New page: ==Bad News== The primers that we ordered for the Purple Russian and the Blue Ox that where supposed to contain the prefix and suffix do not contain a suffix. Sorry about that should have b...) |
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==Volunteer Questions From Saturday== | ==Volunteer Questions From Saturday== | ||
| - | 1. We really don't know why our gel purifications have such low concentrations, today we will run some tests to check and make sure our kit is still good | + | 1. We really don't know why our gel purifications have such low concentrations, today we will run some tests to check and make sure our kit is still good<br> |
| - | 2.The reason I didn't not say to run a gel was because i was afriad that something was wrong with our purifying kit and I thought that we might just decrease our concintration more. Today however James corrected me saying that we should have run 10% of the digest saving the other 90% for ligation. | + | 2.The reason I didn't not say to run a gel was because i was afriad that something was wrong with our purifying kit and I thought that we might just decrease our concintration more. Today however James corrected me saying that we should have run 10% of the digest saving the other 90% for ligation.<br> |
| - | 3. In this PCR we used PFU instead of the Homemade taq making our PCR more reliable and less error prone. | + | 3. In this PCR we used PFU instead of the Homemade taq making our PCR more reliable and less error prone.<br> |
| - | 4. The maps of all vectors are up on the wiki in the parts registry. | + | 4. The maps of all vectors are up on the wiki in the parts registry.<br> |
Revision as of 15:39, 2 June 2008
Bad News
The primers that we ordered for the Purple Russian and the Blue Ox that where supposed to contain the prefix and suffix do not contain a suffix. Sorry about that should have been double checked
Volunteer Questions From Saturday
1. We really don't know why our gel purifications have such low concentrations, today we will run some tests to check and make sure our kit is still good
2.The reason I didn't not say to run a gel was because i was afriad that something was wrong with our purifying kit and I thought that we might just decrease our concintration more. Today however James corrected me saying that we should have run 10% of the digest saving the other 90% for ligation.
3. In this PCR we used PFU instead of the Homemade taq making our PCR more reliable and less error prone.
4. The maps of all vectors are up on the wiki in the parts registry.
