Team:The University of Alberta/Parts

From 2008.igem.org

(Difference between revisions)
(→Butanol Parts)
 
(10 intermediate revisions not shown)
Line 1: Line 1:
{|  align="center"
{|  align="center"
-
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta}} https://static.igem.org/mediawiki/2008/9/9a/Home.png]</span>
+
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta}} https://static.igem.org/mediawiki/2008/a/af/HOMEUAB.png]</span>
-
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Team}} https://static.igem.org/mediawiki/2008/2/2a/Team1.png]</span>
+
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Team}} https://static.igem.org/mediawiki/2008/7/7c/TEAMUA.png]</span>
-
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Project}} https://static.igem.org/mediawiki/2008/9/92/Project.png]</span>
+
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Project}} https://static.igem.org/mediawiki/2008/7/7e/PROJECTUA.png]</span>
-
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Parts}} https://static.igem.org/mediawiki/2008/7/79/Parts.png]</span>
+
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Parts}} https://static.igem.org/mediawiki/2008/c/c0/PARTSUA.png]</span>
-
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Modeling}} https://static.igem.org/mediawiki/2008/3/3c/Modeling.png]</span>
+
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Notebook}} https://static.igem.org/mediawiki/2008/f/f9/NOTEBOOKUA.png]</span>
-
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Notebook}} https://static.igem.org/mediawiki/2008/9/95/Notebook.png]</span>
+
<br>
<br>
<br>
<br>
Line 12: Line 11:
[[image:BioBrick.jpg]]
[[image:BioBrick.jpg]]
|
|
-
==Plant Parts==
 
-
[[Image:Plant_Banner.jpg]]
+
==University of Alberta Parts Registry==
-
*Plant CAMV 35S Promoter
+
<h4>'''All our submitted parts can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=University_of_Alberta in our section of the Parts Registry]!'''</h4> More detailed documentation can be found there for each part.
-
*Plant Resistance Brick
+
-
**Chloramphenicol was used
+
-
*IGEM Binary Vector
+
-
**We constructed the IGEM binary vector using P0380 as a starting point. We removed the PUC8 Multiple cloning site from this binary vector and supplemented our own which will allow the vector to accept biobricks.
+
-
*Blue OX, Purple Russian reporter systems
+
==Plastic Project==
==Plastic Project==
Line 27: Line 20:
[[Image:Plastic_Banner.jpg]]
[[Image:Plastic_Banner.jpg]]
* TetR + LVA tail[http://partsregistry.org/Part:BBa_C0040|]
* TetR + LVA tail[http://partsregistry.org/Part:BBa_C0040|]
-
* PLAC + ERE brick - [[Team:The_University_of_Alberta/Parts/Plastic/lacI_ERE_sequence|Sequence]]
+
* PLACIQ + ERE brick - [[Team:The_University_of_Alberta/Parts/Plastic/lacI_ERE_sequence|Sequence]]
* Estrogen Receptor Alpha (Estrogen binding domain)
* Estrogen Receptor Alpha (Estrogen binding domain)
* Estrogen Receptor Alpha (DNA binding domain)
* Estrogen Receptor Alpha (DNA binding domain)
Line 45: Line 38:
*[[Media:iGEM08_TDNA_MCS.gb]]  
*[[Media:iGEM08_TDNA_MCS.gb]]  
*[[Media:iGEM08_TetR_RBS_6XHis.gb]]
*[[Media:iGEM08_TetR_RBS_6XHis.gb]]
 +
 +
 +
==Plant Parts==
 +
 +
[[Image:Plant_Banner.jpg]]
 +
 +
*Plant CAMV 35S Promoter
 +
*Plant Resistance Brick
 +
**Chloramphenicol was used
 +
*IGEM Binary Vector
 +
**We constructed the IGEM binary vector using P0380 as a starting point. We removed the PUC8 Multiple cloning site from this binary vector and supplemented our own which will allow the vector to accept biobricks.
 +
*Blue OX, Purple Russian reporter systems
 +
 +
 +
==Butanol Parts==
 +
 +
<h4> BioBricks Used This Summer</h4>
 +
 +
* [http://partsregistry.org/Part:BBa_I725021 RBS + B-hydroxy butyryl CoA dehydrogenase] (AKA. '''21''')
 +
* [http://partsregistry.org/Part:BBa_I725022 RBS + Enoyl-CoA hydratase] (AKA. '''22''')
 +
* [http://partsregistry.org/Part:BBa_I725023 RBS + Butyryl CoA dehydrogenase] (AKA. '''23''')
 +
* [http://partsregistry.org/Part:BBa_I725024 RBS + Butyraldehyde dehydrogenase] (AKA. '''24''')
 +
* [http://partsregistry.org/Part:BBa_I725025 RBS + Butanol dehydrogenase] (AKA. '''25''')
 +
* [http://partsregistry.org/Part:BBa_J61003 J61003] (without GFP)
 +
* [http://partsregistry.org/Part:BBa_I0500 I0500]
 +
* [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
 +
<h4>Parts Submitted</h4>
 +
 +
* [http://partsregistry.org/Part:BBa_S04142 21 + 22]  (in plasmid pSB1A3)
 +
* [http://partsregistry.org/Part:BBa_S04145 21 + 22 + 23] (in plasmid pSB1A3)
 +
* [http://partsregistry.org/Part:BBa_S04143 23 + 24] (in plasmid pSB1A3)
 +
* [http://partsregistry.org/Part:BBa_S04144 24 + 25] (in plasmid pSB1A3)
 +
* [http://partsregistry.org/Part:BBa_S04147 21 + 22 + 23 + 24 + 25] (in plasmid pSB1A3)
 +
 +
<h4>Parts Ordered This Summer</h4>
 +
 +
*ETFA  (electron-transfer-flavoprotein, alpha polypeptide)
 +
*ETFB  (electron-transfer-flavoprotein, beta polypeptide)
 +
 +
<h4>Parts Assembled This Summer</h4>
 +
<h5>For Protein Expression</h5>
 +
* J61003 + 21
 +
* J61003 + 22
 +
* J61003 + 23
 +
* J61003 + 24
 +
* J61003 + 25
 +
* I0500 + 21
 +
* I0500 + 22
 +
* I0500 + 23
 +
* I0500 + 24
 +
* I0500 + 25
 +
 +
<h5>For Butanol Operon</h5>
 +
* 21 + 22  (in plasmid pSB1A3)
 +
* 21 + 22 + 23 (in plasmid pSB1A3)
 +
* 23 + 24 (in plasmid pSB1A3)
 +
* 24 + 25 (in plasmid pSB1A3)
 +
* 21 + 22 + 23 + 24 + 25 (in plasmid pSB1A3)
 +
* ETFA + ETFB (in plasmid Puc57)

Latest revision as of 01:35, 30 October 2008

HOMEUAB.pngTEAMUA.pngPROJECTUA.pngPARTSUA.pngNOTEBOOKUA.png

BioBrick.jpg

Contents

University of Alberta Parts Registry

All our submitted parts can be found in our section of the Parts Registry!

More detailed documentation can be found there for each part.

Plastic Project

Plastic Banner.jpg

  • TetR + LVA tail[1]
  • PLACIQ + ERE brick - Sequence
  • Estrogen Receptor Alpha (Estrogen binding domain)
  • Estrogen Receptor Alpha (DNA binding domain)
  • BisdA BPA degredation bio brick - Sequence
  • BisdB BPA degredation bio brick - Sequence

Parts Ordered

PDF Sequences of synthetic genes ordered 20 Jun

Genbank files synthetic genes ordered 20 Jun


Plant Parts

Plant Banner.jpg

  • Plant CAMV 35S Promoter
  • Plant Resistance Brick
    • Chloramphenicol was used
  • IGEM Binary Vector
    • We constructed the IGEM binary vector using P0380 as a starting point. We removed the PUC8 Multiple cloning site from this binary vector and supplemented our own which will allow the vector to accept biobricks.
  • Blue OX, Purple Russian reporter systems


Butanol Parts

BioBricks Used This Summer

Parts Submitted

Parts Ordered This Summer

  • ETFA (electron-transfer-flavoprotein, alpha polypeptide)
  • ETFB (electron-transfer-flavoprotein, beta polypeptide)

Parts Assembled This Summer

For Protein Expression
  • J61003 + 21
  • J61003 + 22
  • J61003 + 23
  • J61003 + 24
  • J61003 + 25
  • I0500 + 21
  • I0500 + 22
  • I0500 + 23
  • I0500 + 24
  • I0500 + 25
For Butanol Operon
  • 21 + 22 (in plasmid pSB1A3)
  • 21 + 22 + 23 (in plasmid pSB1A3)
  • 23 + 24 (in plasmid pSB1A3)
  • 24 + 25 (in plasmid pSB1A3)
  • 21 + 22 + 23 + 24 + 25 (in plasmid pSB1A3)
  • ETFA + ETFB (in plasmid Puc57)
Retrieved from "http://2008.igem.org/Team:The_University_of_Alberta/Parts"