Team:Tokyo Tech/Genetic toggle switch to implement rewritable function

From 2008.igem.org

(Difference between revisions)
Line 4: Line 4:
== Genetic circuit model ==
== Genetic circuit model ==
[[Image:Toggle_genetic_circuit_model.png]]
[[Image:Toggle_genetic_circuit_model.png]]
-
1.
 
-
2.
+
fig5-1:genetic circuit model
 +
 
 +
 
 +
1. '''Writable function'''
 +
 
 +
・ Plac is induced under 30MPa pressure(PL is weakly induced)
 +
 
 +
・ Express CI and GFP
 +
 
 +
・ CI represses PL and causes low LacI concentration
 +
 
 +
・ Express increasingly CI and GFP ⇒ '''Bright!!'''
 +
 
 +
 
 +
2. '''Erasable function'''
 +
 
 +
・PL is induced by heat
 +
 
 +
・Express LacI and LacI represses Plac
 +
 
 +
・Therefore, GFP expression decreases
-
3.
 
== Mathematical model ==
== Mathematical model ==

Revision as of 20:21, 22 October 2008

Genetic toggle switch to implement rewritable function

We think two type models - genetic circuit model and mathematical model.

Genetic circuit model

Toggle genetic circuit model.png

fig5-1:genetic circuit model


1. Writable function

・ Plac is induced under 30MPa pressure(PL is weakly induced)

・ Express CI and GFP

・ CI represses PL and causes low LacI concentration

・ Express increasingly CI and GFP ⇒ Bright!!


2. Erasable function

・PL is induced by heat

・Express LacI and LacI represses Plac

・Therefore, GFP expression decreases


Mathematical model