Team:Tsinghua

From 2008.igem.org

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Our  Team
Our  Team
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                [[Image:Girls' Day 3.jpg]]
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      [[Image:Girls' Day 3.jpg]]
==Team Members ==
==Team Members ==

Revision as of 11:15, 28 July 2008

Although this page is still in heavy construction, every guest is welcomed to left your trace on the "Doodle Board".

             Our Team       Project   Parts      Experiments        Communication Links     Doodle Board

Our Team

      Girls' Day 3.jpg

Team Members

      Luying Jia  Luying Jia.jpg                    Yuanfan Yang 
      Gechong Ruan                Shan Lin
      Xin Rong                       Yicheng LongYicheng Long.jpg
      Ziying Liu                       Yuemeng Wang Yuemeng Wang.jpg
      Yilong Zou Yilong Zou.jpg                    Zi WangZi Wang.jpg
      He YangHe Yang.jpg
      Yongqiang Gou                 Chao WangChao Wang.jpg
      Junjie Luo                    Cong LiuCong Liu.jpg


Project

  This year we are trying to work at the motility of bacteria. E.coli senses some chemicals and moves toward or stays still, this behavior is called chemotaxis. Any modification of the pathway from the sensor to the motile apparatus will cause into different chemotactic effcts.


Parts

Communication

Experiment and Protocol

   1   PCR
    
   1.1 Gold Faster 	Taq system:
   
   Reagent	           Concentration/Activity	50ul 	100ul
   GF taq buffer     	    10x	                         5       10
   GF taq		                                 0.5	  0.5
   dNTPmix	            10mM each	                 1	  2
   Primer 1 	            10uM	                 1	  2
   Primer 2	            10um	                 1	  2
   Template DNA	      changeable	                 0.5	  1
   MgCl2	            0.2M	                 0.5	  1
   ddH2O	                ---	                40.5	 81


   1.2 The program under Gold faster taq system
   Progress	     Program I	                 Program 2
   Predenaturing    95℃         2-5 min	         95℃   2-5 min
   Denaturing	     95℃        10-20sec	         95℃   10-20sec
   Annealing	     (Tm-5) ℃   2-5 sec	         68℃   10-15sec/1kb
   Extension	     72℃        10-15sec/1kb	

25-30cycle 25-30cycle

   Last extension	  1-2min or skipped	       1-2min or skipped
   2 	Restriction cut:
           Reagent	        Concentration/Activity	 Volume(50ul system)
           Restriction cut buffer	10x	           5ul
           Enzyme 1	                --	           2.5ul
           Enzyme 2	                --	           2.5ul
 Incubate at 37℃, 1.5 hrs or longer
 (Enzymes from Takara Co., Ltd)
     3  Ligation:
              Reagent	    Volume(10ul system)
              Solution I	5ul
              DNA fragment	3.5ul
              Vector	        1.5ul
   Incubate at 16-18℃,1hr or longer
   (Ligation kit from Takara)




Links

 Tsinghua University: http://www.tsinghua.edu.cn/qhdwzy/index.jsp
 Parts: http://partsregistry.org
 iGEM Headquarter: hq@igem.org
 Beijing Normal University:https://2008.igem.org/Team:Beijing_Normal 
 ETH_Zurich:https://2008.igem.org/Team:ETH_Zurich
 Nature:http://www.nature.com
 Science:http://www.sciencemag.org
 System and Synthetic Biology:http://www.springer.com/biomed/journal/11693 



Doodle Board

 Oh, My E.coli, where are you!        Yilong 08.7.11