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Team:UCSF/Synthetic Chromatin Properties
From 2008.igem.org
Design of our System (previous)
Synthetic Chromatin Bit (Part II)
Why use Chromatin as a Tool for Synthetic Biology?
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Analysis of our Data
Single-cell analysis was done using flow-cytometry. Flow cytometry measurements were taken using a BD LSR-II flow cytometer (BD Biosciences). For each sample, 10,000 cells were counted, and GFP fluorescence was measured by exciting at 488 nm with a 100 mW Coherent Sapphire laser. Cells that were positive for GFP are seen in the microscope images on the lower right panel and their predicted population distribution is shown in the green curve in the ficticious graph in the left (below).
The Properties of Our Synthetic Chromatin Bit
We studied how our yeast synthetic chromatin system behaves...
1. Targeting of Sir2 leads to complete silencing of reporter
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RESULT 1:
Similar results were obtained for other promoters (e.g. Fig1 P, see below).
2. Dominant over Transcription Factors
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RESULT 2:
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3. Regional Silencing
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RESULT 3:
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4. Binary
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RESULT 4:
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5. Memory
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RESULT 5:
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