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Team:UC Berkeley/GatewayPhagemid
From 2008.igem.org
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==Introduction== | ==Introduction== | ||
| + | The phagemid scheme was motivated by a desire to further streamline the protocol for performing Gateway ''in vivo''. Like the genomic scheme, this method utilizes positive selection to isolate the desired product. However, it improves on this scheme because the lysis and infection steps replace mini-preps and transformations without requiring additional devices. | ||
| + | |||
==Details about Plasmids and Strains== | ==Details about Plasmids and Strains== | ||
==General Procedure== | ==General Procedure== | ||
Revision as of 02:50, 30 October 2008
Phagemid Based Gateway
Introduction
The phagemid scheme was motivated by a desire to further streamline the protocol for performing Gateway in vivo. Like the genomic scheme, this method utilizes positive selection to isolate the desired product. However, it improves on this scheme because the lysis and infection steps replace mini-preps and transformations without requiring additional devices.
Details about Plasmids and Strains
General Procedure
General Phagemid Mechanisms
Conclusion
