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Team:UC Berkeley/GatewayPlasmid
From 2008.igem.org
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== '''Scheme 1: Gateway Machinery in Assembly Vector '''== | == '''Scheme 1: Gateway Machinery in Assembly Vector '''== | ||
| - | Two different variations of the pK112128 assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} between the ccdB site and the attR2 site. These plasmids are named pK112245 and pK112246 respectively (see images below). <br> | + | '''Assembly Vectors:'''Two different variations of the pK112128 assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} between the ccdB site and the attR2 site. These plasmids are named pK112245 and pK112246 respectively (see images below). <br> |
[[Image:pK112245.png]] <br> | [[Image:pK112245.png]] <br> | ||
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| - | The entry vector used in both cases was pBca1256 (see image below).<br> | + | '''Entry Vector:'''The entry vector used in both cases was pBca1256 (see image below).<br> |
[[Image:pBca 1256.png]] <br> | [[Image:pBca 1256.png]] <br> | ||
== '''Scheme 2: Gateway Machinery in Entry Vector '''== | == '''Scheme 2: Gateway Machinery in Entry Vector '''== | ||
| - | The assembly vector used for each of the following entry vector variations is pK112128 (see image below. <br> | + | '''Assembly Vector:'''The assembly vector used for each of the following entry vector variations is pK112128 (see image below. <br> |
[[Image:pK112128.png]] <br> | [[Image:pK112128.png]] <br> | ||
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| - | Two different variations of the pBca1256 entry vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} just before the attL1 site. These plasmids are named pK112247 and pK112248 respectively (see images below). | + | '''Entry Vectors:''' Two different variations of the pBca1256 entry vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} just before the attL1 site. These plasmids are named pK112247 and pK112248 respectively (see images below).<br> |
[[Image:pK112247.png]] <br> | [[Image:pK112247.png]] <br> | ||
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[[Image:pK112248.png]] <br> | [[Image:pK112248.png]] <br> | ||
Revision as of 04:16, 28 October 2008
Gateway Plasmid Schemes
Scheme 1: Gateway Machinery in Assembly Vector
Assembly Vectors:Two different variations of the pK112128 assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} between the ccdB site and the attR2 site. These plasmids are named pK112245 and pK112246 respectively (see images below).
Entry Vector:The entry vector used in both cases was pBca1256 (see image below).
Scheme 2: Gateway Machinery in Entry Vector
Assembly Vector:The assembly vector used for each of the following entry vector variations is pK112128 (see image below.
Entry Vectors: Two different variations of the pBca1256 entry vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} just before the attL1 site. These plasmids are named pK112247 and pK112248 respectively (see images below).
