http://2008.igem.org/wiki/index.php?title=Team:UC_Berkeley/GatewayPlasmid&feed=atom&action=historyTeam:UC Berkeley/GatewayPlasmid - Revision history2024-03-28T17:24:22ZRevision history for this page on the wikiMediaWiki 1.16.5http://2008.igem.org/wiki/index.php?title=Team:UC_Berkeley/GatewayPlasmid&diff=103367&oldid=prevJinism83: /* Gateway Device in Assembly Vector: Plasmid Details */2008-10-30T03:33:10Z<p><span class="autocomment">Gateway Device in Assembly Vector: Plasmid Details</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Assembly Vector: Plasmid Details '''==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Assembly Vector: Plasmid Details '''==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Assembly Vectors:'''Two different variations of the pK112128 assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfα!}{rbs.ihfβ!} between the ccdB site and the attR2 site. These plasmids are named [https://static.igem.org/mediawiki/2008/b/b3/PK112245.png pK112245] and [https://static.igem.org/mediawiki/2008/f/f3/PK112246.png pK112246] respectively. A simplified version of the two variants along with the Lysis Device is shown below. <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Assembly Vectors:'''Two different variations of the pK112128 assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfα!}{rbs.ihfβ!} between the ccdB site and the attR2 site. These plasmids are named [https://static.igem.org/mediawiki/2008/b/b3/PK112245.png pK112245] and [https://static.igem.org/mediawiki/2008/f/f3/PK112246.png pK112246] respectively. A simplified version of the two variants along with the Lysis Device is shown below. <ins class="diffchange diffchange-inline"><br></ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof245or246.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof245or246.png]] <br></div></td></tr>
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</table>Jinism83http://2008.igem.org/wiki/index.php?title=Team:UC_Berkeley/GatewayPlasmid&diff=102607&oldid=prevAronlau: /* Gateway Device in Entry Vector: Plasmid Details */2008-10-30T03:07:17Z<p><span class="autocomment">Gateway Device in Entry Vector: Plasmid Details</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Entry Vectors:''' Two different variations of the pBca1256 entry vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfα!}{rbs.ihfβ!} just before the attL1 site. These plasmids are named [https://static.igem.org/mediawiki/2008/2/2d/PK112247.png pK112247] and [https://static.igem.org/mediawiki/2008/a/ae/PK112248.png pK112248] respectively <del class="diffchange diffchange-inline">(see links below)</del>.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Entry Vectors:''' Two different variations of the pBca1256 entry vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfα!}{rbs.ihfβ!} just before the attL1 site. These plasmids are named [https://static.igem.org/mediawiki/2008/2/2d/PK112247.png pK112247] and [https://static.igem.org/mediawiki/2008/a/ae/PK112248.png pK112248] respectively.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof247or248.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof247or248.png]] <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
</table>Aronlauhttp://2008.igem.org/wiki/index.php?title=Team:UC_Berkeley/GatewayPlasmid&diff=102585&oldid=prevAronlau: /* Gateway Device in Assembly Vector: Plasmid Details */2008-10-30T03:06:28Z<p><span class="autocomment">Gateway Device in Assembly Vector: Plasmid Details</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Entry Vector:'''The entry vector used in both cases was pBca1256 <del class="diffchange diffchange-inline">(see image and link below)</del>.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Entry Vector:'''The entry vector used in both cases was <ins class="diffchange diffchange-inline">[https://static.igem.org/mediawiki/2008/8/85/PBca_1256.png </ins>pBca1256<ins class="diffchange diffchange-inline">]</ins>.<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[[media:pBca 1256.png]] </del><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof1256.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof1256.png]] <br></div></td></tr>
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</table>Aronlauhttp://2008.igem.org/wiki/index.php?title=Team:UC_Berkeley/GatewayPlasmid&diff=102562&oldid=prevAronlau: /* Gateway Device in Assembly Vector: Plasmid Details */2008-10-30T03:05:43Z<p><span class="autocomment">Gateway Device in Assembly Vector: Plasmid Details</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Assembly Vector: Plasmid Details '''==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Assembly Vector: Plasmid Details '''==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Assembly Vectors:'''Two different variations of the pK112128 assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfα!}{rbs.ihfβ!} between the ccdB site and the attR2 site. These plasmids are named pK112245 and pK112246 respectively <del class="diffchange diffchange-inline">(see image links below)</del>. A simplified version of the two variants along with the Lysis Device is shown below. <del class="diffchange diffchange-inline"><br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Assembly Vectors:'''Two different variations of the pK112128 assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfα!}{rbs.ihfβ!} between the ccdB site and the attR2 site. These plasmids are named <ins class="diffchange diffchange-inline">[https://static.igem.org/mediawiki/2008/b/b3/PK112245.png </ins>pK112245<ins class="diffchange diffchange-inline">] </ins>and <ins class="diffchange diffchange-inline">[https://static.igem.org/mediawiki/2008/f/f3/PK112246.png </ins>pK112246<ins class="diffchange diffchange-inline">] </ins>respectively. A simplified version of the two variants along with the Lysis Device is shown below. <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[[media:pK112245.png]]<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[[media:pK112246.png]]</del><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof245or246.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof245or246.png]] <br></div></td></tr>
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</table>Aronlauhttp://2008.igem.org/wiki/index.php?title=Team:UC_Berkeley/GatewayPlasmid&diff=102522&oldid=prevAronlau: /* Gateway Device in Entry Vector: Plasmid Details */2008-10-30T03:04:27Z<p><span class="autocomment">Gateway Device in Entry Vector: Plasmid Details</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Entry Vectors:''' Two different variations of the pBca1256 entry vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfα!}{rbs.ihfβ!} just before the attL1 site. These plasmids are named [https://static.igem.org/mediawiki/2008/2/2d/PK112247.png pK112247] and pK112248 respectively (see links below).<del class="diffchange diffchange-inline"><br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Entry Vectors:''' Two different variations of the pBca1256 entry vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfα!}{rbs.ihfβ!} just before the attL1 site. These plasmids are named [https://static.igem.org/mediawiki/2008/2/2d/PK112247.png pK112247] and <ins class="diffchange diffchange-inline">[https://static.igem.org/mediawiki/2008/a/ae/PK112248.png </ins>pK112248<ins class="diffchange diffchange-inline">] </ins>respectively (see links below).<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[[media:pK112247.png]] <br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[[media:pK112248.png]] </del><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof247or248.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof247or248.png]] <br></div></td></tr>
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</table>Aronlauhttp://2008.igem.org/wiki/index.php?title=Team:UC_Berkeley/GatewayPlasmid&diff=102513&oldid=prevAronlau: /* Gateway Device in Entry Vector: Plasmid Details */2008-10-30T03:04:03Z<p><span class="autocomment">Gateway Device in Entry Vector: Plasmid Details</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Entry Vectors:''' Two different variations of the pBca1256 entry vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfα!}{rbs.ihfβ!} just before the attL1 site. These plasmids are named pK112247 and pK112248 respectively (see links below).<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Entry Vectors:''' Two different variations of the pBca1256 entry vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfα!}{rbs.ihfβ!} just before the attL1 site. These plasmids are named <ins class="diffchange diffchange-inline">[https://static.igem.org/mediawiki/2008/2/2d/PK112247.png </ins>pK112247<ins class="diffchange diffchange-inline">] </ins>and pK112248 respectively (see links below).<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[media:pK112247.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[media:pK112247.png]] <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[media:pK112248.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[media:pK112248.png]] <br></div></td></tr>
</table>Aronlauhttp://2008.igem.org/wiki/index.php?title=Team:UC_Berkeley/GatewayPlasmid&diff=102493&oldid=prevAronlau: /* Gateway Device in Entry Vector: Plasmid Details */2008-10-30T03:03:14Z<p><span class="autocomment">Gateway Device in Entry Vector: Plasmid Details</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Entry Vector: Plasmid Details'''==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Entry Vector: Plasmid Details'''==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Assembly Vector:'''The assembly vector used for each of the following entry vector variations is [https://static.igem.org/mediawiki/2008/7/76/PK112128.png pK112128] <del class="diffchange diffchange-inline">(see link and image below</del>. <br> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Assembly Vector:'''The assembly vector used for each of the following entry vector variations is [https://static.igem.org/mediawiki/2008/7/76/PK112128.png pK112128]. <br> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[[media:pK112128.png]] <br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof128.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof128.png]] <br></div></td></tr>
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</table>Aronlauhttp://2008.igem.org/wiki/index.php?title=Team:UC_Berkeley/GatewayPlasmid&diff=102438&oldid=prevAronlau: /* Gateway Device in Entry Vector: Plasmid Details */2008-10-30T03:01:15Z<p><span class="autocomment">Gateway Device in Entry Vector: Plasmid Details</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Entry Vector: Plasmid Details'''==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Entry Vector: Plasmid Details'''==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Assembly Vector:'''The assembly vector used for each of the following entry vector variations is pK112128 (see link and image below. <br> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Assembly Vector:'''The assembly vector used for each of the following entry vector variations is <ins class="diffchange diffchange-inline">[https://static.igem.org/mediawiki/2008/7/76/PK112128.png </ins>pK112128<ins class="diffchange diffchange-inline">] </ins>(see link and image below. <br> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[media:pK112128.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[media:pK112128.png]] <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof128.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof128.png]] <br></div></td></tr>
</table>Aronlauhttp://2008.igem.org/wiki/index.php?title=Team:UC_Berkeley/GatewayPlasmid&diff=102421&oldid=prevAronlau: /* Gateway Device in Entry Vector: Plasmid Details */2008-10-30T03:00:47Z<p><span class="autocomment">Gateway Device in Entry Vector: Plasmid Details</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 03:00, 30 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Entry Vector: Plasmid Details'''==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Entry Vector: Plasmid Details'''==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Assembly Vector:'''The assembly vector used for each of the following entry vector variations is <del class="diffchange diffchange-inline">[https://static.igem.org/mediawiki/2008/2/2d/PK112247.png|</del>pK112128<del class="diffchange diffchange-inline">] </del>(see link and image below. <br> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Assembly Vector:'''The assembly vector used for each of the following entry vector variations is pK112128 (see link and image below. <br> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[media:pK112128.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[media:pK112128.png]] <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof128.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof128.png]] <br></div></td></tr>
</table>Aronlauhttp://2008.igem.org/wiki/index.php?title=Team:UC_Berkeley/GatewayPlasmid&diff=102413&oldid=prevAronlau: /* Gateway Device in Entry Vector: Plasmid Details */2008-10-30T03:00:22Z<p><span class="autocomment">Gateway Device in Entry Vector: Plasmid Details</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 03:00, 30 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Entry Vector: Plasmid Details'''==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Gateway Device in Entry Vector: Plasmid Details'''==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Assembly Vector:'''The assembly vector used for each of the following entry vector variations is [https://static.igem.org/mediawiki/2008/2/2d/PK112247.png| pK112128] (see link and image below. <br> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Assembly Vector:'''The assembly vector used for each of the following entry vector variations is [https://static.igem.org/mediawiki/2008/2/2d/PK112247.png|pK112128] (see link and image below. <br> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[media:pK112128.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[media:pK112128.png]] <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof128.png]] <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:simpof128.png]] <br></div></td></tr>
</table>Aronlau