Team:UC Berkeley/Notebook/Aron Lau/material

From 2008.igem.org

(Difference between revisions)
Line 1: Line 1:
{{UCBmain}}
{{UCBmain}}
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===Blocking Solution (500 mL)===
+
==Blocking Solution (500 mL)==
  50mL 10x PBS
  50mL 10x PBS
  5g BSA
  5g BSA
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  ddH20 to 500mL
  ddH20 to 500mL
-
===Wash Solution(1L)===
+
==Wash Solution(1L)==
  100mL 10x PBs
  100mL 10x PBs
  500ul tween
  500ul tween
  ddH20 to 1L
  ddH20 to 1L

Revision as of 01:23, 26 October 2008

Blocking Solution (500 mL)

50mL 10x PBS
5g BSA
250ul tween
ddH20 to 500mL

Wash Solution(1L)

100mL 10x PBs
500ul tween
ddH20 to 1L