Team:UNIPV-Pavia/Notebook/Week1

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Revision as of 18:13, 27 May 2008

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Notebook

Week 1: 05/19/08 - May 05/23/08

05/19/08

• Let’s start our IGEM 2008 experience! At first, we broke the punch tool…:)

• We used a scalpel to cut and resuspend the following 22 paper spots:

• We used tweezers to put the cut paper into tubes containing 10 μl of warmed TE buffer.

• We transformed 60 µl of TOP10 E. coli with 4 µl of DNA in TE for all 22 parts, plated transformed bacteria and incubated overnight at 37°C.

• We used LB medium previously prepared, with the suitable antibiotic added.

05/20/08

• After overnight incubation, the following 14 plates showed colonies:

BBa_E0240

• While the following plates did not:

• Plate containing BBa_J23100 showed red colonies, as we expected: this part is inserted into plasmid BBa_J61002 which places the RFP downstream of the inserted part, which is a constitutive promoter.

• We picked up one colony from every working plate to grow 5 ml cultures of transformed bacteria overnight.

• We re-transformed 60 µl of TOP10 with the remaining 6 µl of DNA in TE for BBa_I14032, BBa_R0051, BBa_I15008, BBa_I15009, BBa_I15010, BBa_C0161, BBa_C0078, BBa_C0179.

• We plated transformed bacteria and incubated them at 37°C overnight.

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