Team:University of Alberta/Plant Project

From 2008.igem.org

(Difference between revisions)
Line 1: Line 1:
-
'''First Seed Pod of the project'''<br>
+
==Introduction==
-
[[image:first seed pods.jpg|200px]]
+
To date, synthetic biology has focused largely on single cell organisms.  The goal of our project is to build BioBrick to facilitate application of synthetic biology principles to the genetic engineering of higher plants.  We are developing standardized, open source binary vectors, promoters, and resistance genes for use in plants, each of which is to conform to BioBrick specifications.  The application of the BioBricks in transformation of protoplasts and whole plants of tobacco and Arabidopsis, respectively, will be demonstrated, as well as a general strategy for increasing the general availability of synthetic biology tools for plant genetic engineering.
-
 
+
== IGEM's First Binary Vector ==
== IGEM's First Binary Vector ==
[[image:Binary Vector.png]]
[[image:Binary Vector.png]]
Line 8: Line 7:
We have added in our own poly linker which includes the proper prefix, suffix and primers to coincide with the IGEM regulations. We also added a CAM resistance cassette.
We have added in our own poly linker which includes the proper prefix, suffix and primers to coincide with the IGEM regulations. We also added a CAM resistance cassette.
-
==The First Plant Project Ever Submitted to IGEM==
+
==The IGEM Plant Project==
We hope to demonstrate that using biobricks in plants is not only useful but feasible in the restraints of this competition. We also hope to use the tools that we make to insert our toxin detection vector into plants.
We hope to demonstrate that using biobricks in plants is not only useful but feasible in the restraints of this competition. We also hope to use the tools that we make to insert our toxin detection vector into plants.
Line 16: Line 15:
[[Transformation Protocol]]
[[Transformation Protocol]]
-
==Time line==
+
==Status of Project==
-
A new tray of plants will be planted every week.
+
Currently we have the multiple cloning site (MCS) and the vector that we are introducing it into (p0380). We have optimized the vector p0380 using site directed mutagenesis to remove all pre-existing notI sites from the vector. At the moment we are in the process of verifying that our MCS has been successfully inserted into the vector.
-
'''Week 1'''(Before Biobricks Arrive)<br>
+
'''First Seed Pod of the project'''<br>
-
PCR our the resistance gene and promoter from the Pcambia vector<br>
+
[[image:first seed pods.jpg|200px]]
-
Use Site directed mutagenesis to remove NOTI sites from the vector<br>
+
-
'''Week 2'''(biobricks have arrived)<br>
 
-
Digest new biobrick and confirm it is what it is.<br>
 
-
Insert Plant biobrick into the pCambia vector that has had the NOTI sites removed<br>
 
-
Transform Vector into Agrobacterium<br>
 
-
Confirm Transformation<br>
 
-
Create bulk culture of Agrobacterium for plant transformation
 
-
 
-
'''Week 3'''
 
-
Transform Plants ( That are 3 weeks old)<br>
 
-
Make another bulk culture for next weeks transformation <br>
 
-
'''Week 4-6'''
 
-
Keep transforming plants on tray every week to increase chances.<br>
 
-
Harvest seeds of transformed plants and replant them.(continue as seeds come into maturity)<br>
 
-
'''Week 6-End'''
 
-
select for homozygous plants
 
-
==Status of Project==
 
-
Currently we have the multiple cloning site (MCS) and the vector that we are introducing it into (p0380). We have optimized the vector p0380 using site directed mutagenesis to remove all pre-existing notI sites from the vector. At the moment we are in the process of verifying that our MCS has been successfully inserted into the vector.
 
<br>
<br>
<br>
<br>
<h2>'''Back to [https://2008.igem.org/Team:The_University_of_Alberta/Project the projects page].'''</h2>
<h2>'''Back to [https://2008.igem.org/Team:The_University_of_Alberta/Project the projects page].'''</h2>

Revision as of 03:41, 29 October 2008

Contents

Introduction

To date, synthetic biology has focused largely on single cell organisms. The goal of our project is to build BioBrick to facilitate application of synthetic biology principles to the genetic engineering of higher plants. We are developing standardized, open source binary vectors, promoters, and resistance genes for use in plants, each of which is to conform to BioBrick specifications. The application of the BioBricks in transformation of protoplasts and whole plants of tobacco and Arabidopsis, respectively, will be demonstrated, as well as a general strategy for increasing the general availability of synthetic biology tools for plant genetic engineering.

IGEM's First Binary Vector

Binary Vector.png

Our Binary Vector is a modified pCambia 0380 binary vector [link] We have added in our own poly linker which includes the proper prefix, suffix and primers to coincide with the IGEM regulations. We also added a CAM resistance cassette.

The IGEM Plant Project

We hope to demonstrate that using biobricks in plants is not only useful but feasible in the restraints of this competition. We also hope to use the tools that we make to insert our toxin detection vector into plants.

Plant Protocol

Growing Plants
Selection of Primary Transformants
Transformation Protocol

Status of Project

Currently we have the multiple cloning site (MCS) and the vector that we are introducing it into (p0380). We have optimized the vector p0380 using site directed mutagenesis to remove all pre-existing notI sites from the vector. At the moment we are in the process of verifying that our MCS has been successfully inserted into the vector.

First Seed Pod of the project
First seed pods.jpg



Back to the projects page.

Retrieved from "http://2008.igem.org/Team:University_of_Alberta/Plant_Project"