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Team:University of Chicago/Notebook/Norayucel
From 2008.igem.org
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| - | == | + | ==July 18, 2008== |
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| + | 1. Diluted 1ng/microliter pGreen plasmid to 10pg/microliter with 100X dilution | ||
| + | 2. 1microliter of plasmid to 50 microliters competent cells | ||
| + | 3. Will grow up 50microliters of TOP10 untransformed as a control | ||
| + | 4. Put on ice at 2:03 | ||
| + | 5. Took off ice at 2:34 | ||
| + | 6. Incubated for 1minute at 42C | ||
| + | 7. Added 250microliters SOC media (prepared at 1:30) | ||
| + | 8. Start incubated at 37C at 2:36 | ||
| + | 9. Take out at 3:40pm | ||
| + | 10. Streaked 20microliters onto Amp. Plates | ||
| + | 11. Dan will come in Saturday morning to pick up the plates and count colonies. | ||
Revision as of 21:24, 18 July 2008
July 18, 2008
1. Diluted 1ng/microliter pGreen plasmid to 10pg/microliter with 100X dilution 2. 1microliter of plasmid to 50 microliters competent cells 3. Will grow up 50microliters of TOP10 untransformed as a control 4. Put on ice at 2:03 5. Took off ice at 2:34 6. Incubated for 1minute at 42C 7. Added 250microliters SOC media (prepared at 1:30) 8. Start incubated at 37C at 2:36 9. Take out at 3:40pm 10. Streaked 20microliters onto Amp. Plates 11. Dan will come in Saturday morning to pick up the plates and count colonies.
