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Team:University of Lethbridge/Modeling
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For our project, we decided to create a synthetic bacterium which would move towards and degrade an environmental pollutant. To direct the movement of our modified "Bacuum Cleaner," we reprogrammed the chemotaxis pathway by using a toxin-responsive riboswitch to control expression of the motility protein CheZ. CheZ catalyzes the dephosphorylation of the motility protein CheY, which promotes directed movement (chemotaxis) over the "tumbling" state of the bacterium. | For our project, we decided to create a synthetic bacterium which would move towards and degrade an environmental pollutant. To direct the movement of our modified "Bacuum Cleaner," we reprogrammed the chemotaxis pathway by using a toxin-responsive riboswitch to control expression of the motility protein CheZ. CheZ catalyzes the dephosphorylation of the motility protein CheY, which promotes directed movement (chemotaxis) over the "tumbling" state of the bacterium. | ||
| - | We used the modeling program BCT (ref) to determine the level of ''CheZ'' gene expression necessary | + | We used the modeling program BCT (ref) to determine the level of ''CheZ'' gene expression necessary to produce dephosphorylated CheY and directed movement. Increasing intracellular concentrations of CheZ leads to increased CheY in the unphosphorylated state (Figure 1a)... |
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Revision as of 05:16, 26 October 2008
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[http://2008.igem.org/wiki/index.php?title=Team:University_of_Lethbridge&Home |
[http://2008.igem.org/Team:University_of_Lethbridge/Team |
[http://2008.igem.org/Team:University_of_Lethbridge/Project |
[http://2008.igem.org/Team:University_of_Lethbridge/Parts |
[http://2008.igem.org/Team:University_of_Lethbridge/Modeling |
[http://2008.igem.org/Team:University_of_Lethbridge/Notebook |
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Our Project
For our project, we decided to create a synthetic bacterium which would move towards and degrade an environmental pollutant. To direct the movement of our modified "Bacuum Cleaner," we reprogrammed the chemotaxis pathway by using a toxin-responsive riboswitch to control expression of the motility protein CheZ. CheZ catalyzes the dephosphorylation of the motility protein CheY, which promotes directed movement (chemotaxis) over the "tumbling" state of the bacterium.
We used the modeling program BCT (ref) to determine the level of CheZ gene expression necessary to produce dephosphorylated CheY and directed movement. Increasing intracellular concentrations of CheZ leads to increased CheY in the unphosphorylated state (Figure 1a)...
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| Figure 1(a) | Figure 1(b) |
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[http://2008.igem.org/wiki/index.php?title=Team:University_of_Lethbridge&Home |
[http://2008.igem.org/Team:University_of_Lethbridge/Team |
[http://2008.igem.org/Team:University_of_Lethbridge/Project |
[http://2008.igem.org/Team:University_of_Lethbridge/Parts |
[http://2008.igem.org/Team:University_of_Lethbridge/Modeling |
[http://2008.igem.org/Team:University_of_Lethbridge/Notebook |
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