Team:University of Lethbridge/Notebook/GeneralLabAugust

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(Difference between revisions)
(New page: ===Augest 13, 2008=== ====Nathun Puhl and Sebastian==== Varified and quantified the LacI and DT restriction digest products. Set up a Ligase experiment for LacI and DT --1.3 uL vector(DT...)
(Nathun Puhl and Sebastian)
Line 5: Line 5:
Set up a Ligase experiment for LacI and DT
Set up a Ligase experiment for LacI and DT
-
--1.3 uL vector(DT)
+
- 1.3 uL vector(DT)
-
--8 uL insert(LacI)
+
- 8 uL insert(LacI)
-
--2 uL 10x Ligase buffer
+
- 2 uL 10x Ligase buffer
-
--1.5 uL Ligase
+
- 1.5 uL Ligase
-
--7.2 uL ddH2O
+
- 7.2 uL ddH2O
-
--20 uL total volume
+
- 20 uL total volume

Revision as of 02:09, 14 August 2008

Augest 13, 2008

Nathun Puhl and Sebastian

Varified and quantified the LacI and DT restriction digest products.

Set up a Ligase experiment for LacI and DT

- 1.3 uL vector(DT)
- 8 uL insert(LacI)
- 2 uL 10x Ligase buffer
- 1.5 uL Ligase
- 7.2 uL ddH2O
- 20 uL total volume