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Team:University of Lethbridge/Notebook/GeneralLabJuly
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====Nathan Puhl, Andrew==== | ====Nathan Puhl, Andrew==== | ||
Made 500 mL of LB agar + amp and 500 mL of Liquid LB | Made 500 mL of LB agar + amp and 500 mL of Liquid LB | ||
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===July 2, 2008=== | ===July 2, 2008=== | ||
====Nathan Puhl, Alix, Sebastian, Munima, Roxanne, Christa==== | ====Nathan Puhl, Alix, Sebastian, Munima, Roxanne, Christa==== | ||
| - | + | Transformed BBa_J24679 (RBS + LacI), BBa_P0440 (RBS+TetR+T10+T12), leftover DNA from Double T (June 26, 2008), and 1 uL of pSB1A7 plasmid (June 18, 2008). | |
Protocol changes: | Protocol changes: | ||
-3 uL of DNA | -3 uL of DNA | ||
-spin down 200 uL of cells and resuspend in 100 uL of LB; plate on LB + amp | -spin down 200 uL of cells and resuspend in 100 uL of LB; plate on LB + amp | ||
| + | |||
===July 3, 2008=== | ===July 3, 2008=== | ||
====Nathan Puhl, Munima, Christa, Alix, Roxanne, Sebastian==== | ====Nathan Puhl, Munima, Christa, Alix, Roxanne, Sebastian==== | ||
Checked transformation plates. Only the positive control (pSB1A7) had colonies (~1500) indicating that there is nothing wrong with the transformation protocol or cells so we must be having problems with the DNA extraction from the filter paper. Next week we will attempt various changes to the protocol to extract more DNA. | Checked transformation plates. Only the positive control (pSB1A7) had colonies (~1500) indicating that there is nothing wrong with the transformation protocol or cells so we must be having problems with the DNA extraction from the filter paper. Next week we will attempt various changes to the protocol to extract more DNA. | ||
Revision as of 16:20, 4 July 2008
Contents |
July 1, 2008
Nathan Puhl, Andrew
Made 500 mL of LB agar + amp and 500 mL of Liquid LB
July 2, 2008
Nathan Puhl, Alix, Sebastian, Munima, Roxanne, Christa
Transformed BBa_J24679 (RBS + LacI), BBa_P0440 (RBS+TetR+T10+T12), leftover DNA from Double T (June 26, 2008), and 1 uL of pSB1A7 plasmid (June 18, 2008).
Protocol changes:
-3 uL of DNA -spin down 200 uL of cells and resuspend in 100 uL of LB; plate on LB + amp
July 3, 2008
Nathan Puhl, Munima, Christa, Alix, Roxanne, Sebastian
Checked transformation plates. Only the positive control (pSB1A7) had colonies (~1500) indicating that there is nothing wrong with the transformation protocol or cells so we must be having problems with the DNA extraction from the filter paper. Next week we will attempt various changes to the protocol to extract more DNA.
