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Team:University of Lethbridge/Notebook/GeneralLabJuly
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===July 2, 2008=== | ===July 2, 2008=== | ||
| - | ====Nathan, Alix, Sebastian, Munima, Roxanne, Christa==== | + | ====Nathan Puhl, Alix, Sebastian, Munima, Roxanne, Christa==== |
Trasformed BBa_J24679( RBS + LacI), BBa_P0440 (RBS+TetR+T10+T12), leftover DNA from Double T (June 26, 2008), and 1 uL of pSB1A7 plasmid (June 18). | Trasformed BBa_J24679( RBS + LacI), BBa_P0440 (RBS+TetR+T10+T12), leftover DNA from Double T (June 26, 2008), and 1 uL of pSB1A7 plasmid (June 18). | ||
Revision as of 05:11, 4 July 2008
Contents |
July 1, 2008
Nathan Puhl, Andrew
Made 500 mL of LB agar + amp and 500 mL of Liquid LB
July 2, 2008
Nathan Puhl, Alix, Sebastian, Munima, Roxanne, Christa
Trasformed BBa_J24679( RBS + LacI), BBa_P0440 (RBS+TetR+T10+T12), leftover DNA from Double T (June 26, 2008), and 1 uL of pSB1A7 plasmid (June 18).
Protocol changes:
-3 uL of DNA -spin down 200 uL of cells and resuspend in 100 uL of LB; plate on LB + amp
