Team:University of Lethbridge/Notebook/GeneralLabJune

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Contents

June 6 2008

Sebastian and Roxanne

Prepared 1L of semi-solid media following the procedure found on OpenWetWare.

-10g peptone
-10g Agar
-10g NaCl
-5g Yeast Extract

Semi-solid Media was autoclaved and stored in the iGEM fridge for making plates at a later date.

June 10 2008

Christa, Munima, Roxanne, and Sebastian

Prepared 1L of liquid media following the procedure found on OpenWetWare.

-10g peptone
-10g NaCl
-5g Yeast Extract

36 culture test tubes were poured, and the remainder was left in a 1L Erlenmeyer flask. Both were autoclaved and stored in the iGEM fridge.

Christa

Took and inventory of iGEM 2007 parts in Wieden -80 freezer

June 11 2008

Sebastian, Munima, Roxanne, Christa

Poured 8 minimal media (labeled control- with blue sharpie) plates and 14 ampicillin plates (labeled ampicillin- with red sharpie)with aseptic technique and stored in the 4C fridge. Ampicillin concentration is 50ug/mL according to the Topp and Gallivan paper.