Team:University of Lethbridge/Notebook/Project1June

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Latest revision as of 02:31, 30 October 2008

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 ===June 16, 2008===
 ====Nathan Puhl, Munima, Christa, Sebastian, Roxanne====
 Plated cheZ knockout strain from glycerol on LB + amp and Plain LB to assess viability and antibiotic resistance at 37 C overnight.
 ===June 17 2008===
 ====Munima, Christa, Nathan Puhl====
 cheZ knockout strain viable on LB, no growth on LB + amp - Good
 ===June 30, 2008===
 ====Nathan Puhl, Christa, Munima====
 plated RP1616 and pTopp cells from glycerol stocks onto LB and LB + amp, respectively
-===July 1, 2008===
-====Nathan Puhl====
-Subcultured RP1616 into liquid LB and pTopp cells into liquid LB + amp
-===July 2, 2008===
-====Nathan Puhl, Christa, Munima====
-Plasma mini prepped pTopp cells
-Attempted to make RP1616 cells competent using [http://openwetware.org/wiki/Preparing_chemically_competent_cells].  100 uL aliquots of cells were froxen in liquid nitrogen and stored in the -80 freezer.
-Ran CheZ in pUC19 plasmid and pUC19 plasmid from last year
-[[Image:puc19 + cheZ.jpg]]