Team:University of Lethbridge/Notebook/Project1June

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====Nathan Puhl, Christa, Munima====
====Nathan Puhl, Christa, Munima====
plated RP1616 and pTopp cells from glycerol stocks onto LB and LB + amp, respectively
plated RP1616 and pTopp cells from glycerol stocks onto LB and LB + amp, respectively
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===July 1, 2008===
 
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====Nathan Puhl====
 
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Subcultured RP1616 into liquid LB and pTopp cells into liquid LB + amp
 
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===July 2, 2008===
 
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====Nathan Puhl, Christa, Munima====
 
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Plasma mini prepped pTopp cells
 
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Attempted to make RP1616 cells competent using the open wet ware [http://openwetware.org/wiki/Preparing_chemically_competent_cells protocol].  100 uL aliquots of cells were froxen in liquid nitrogen and stored in the -80 freezer.
 
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Ran CheZ in pUC19 plasmid and pUC19 plasmid from last year with high range ladder.  The pUC19 plasmid seems weird, I don't think it should have two bands; I will have to ask someone about that.  The CheZ plasmid appears to be the right size (2600 + 620 = 3220 bp)
 
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[[Image:puc19 + cheZ.jpg|500 px]]
 

Revision as of 07:34, 3 July 2008

Contents

June 16, 2008

Nathan Puhl, Munima, Christa, Sebastian, Roxanne

Plated cheZ knockout strain from glycerol on LB + amp and Plain LB to assess viability and antibiotic resistance at 37 C overnight.

June 17 2008

Munima, Christa, Nathan Puhl

cheZ knockout strain viable on LB, no growth on LB + amp - Good

June 30, 2008

Nathan Puhl, Christa, Munima

plated RP1616 and pTopp cells from glycerol stocks onto LB and LB + amp, respectively

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