"
Team:University of Lethbridge/Notebook/Project1June
From 2008.igem.org
(Difference between revisions)
Nathan.puhl (Talk | contribs) |
Nathan.puhl (Talk | contribs) |
||
| Line 19: | Line 19: | ||
Plasma mini prepped pTopp cells | Plasma mini prepped pTopp cells | ||
| - | Attempted to make RP1616 cells competent using [http://openwetware.org/wiki/Preparing_chemically_competent_cells]. 100 uL aliquots of cells were froxen in liquid nitrogen and stored in the -80 freezer. | + | Attempted to make RP1616 cells competent using the open wet ware [http://openwetware.org/wiki/Preparing_chemically_competent_cells protocol]. 100 uL aliquots of cells were froxen in liquid nitrogen and stored in the -80 freezer. |
Ran CheZ in pUC19 plasmid and pUC19 plasmid from last year | Ran CheZ in pUC19 plasmid and pUC19 plasmid from last year | ||
| - | [[Image:puc19 + cheZ.jpg]] | + | [[Image:puc19 + cheZ.jpg|500 px]] |
Revision as of 01:13, 3 July 2008
Contents |
June 16, 2008
Nathan Puhl, Munima, Christa, Sebastian, Roxanne
Plated cheZ knockout strain from glycerol on LB + amp and Plain LB to assess viability and antibiotic resistance at 37 C overnight.
June 17 2008
Munima, Christa, Nathan Puhl
cheZ knockout strain viable on LB, no growth on LB + amp - Good
June 30, 2008
Nathan Puhl, Christa, Munima
plated RP1616 and pTopp cells from glycerol stocks onto LB and LB + amp, respectively
July 1, 2008
Nathan Puhl
Subcultured RP1616 into liquid LB and pTopp cells into liquid LB + amp
July 2, 2008
Nathan Puhl, Christa, Munima
Plasma mini prepped pTopp cells
Attempted to make RP1616 cells competent using the open wet ware protocol. 100 uL aliquots of cells were froxen in liquid nitrogen and stored in the -80 freezer.
Ran CheZ in pUC19 plasmid and pUC19 plasmid from last year
