"
Team:University of Lethbridge/Notebook/Project1June
From 2008.igem.org
Contents |
June 16, 2008
Nathan Puhl, Munima, Christa, Sebastian, Roxanne
Plated cheZ knockout strain from glycerol on LB + amp and Plain LB to assess viability and antibiotic resistance at 37 C overnight.
June 17 2008
Munima, Christa, Nathan Puhl
cheZ knockout strain viable on LB, no growth on LB + amp - Good
June 30, 2008
Nathan Puhl, Christa, Munima
plated RP1616 and pTopp cells from glycerol stocks onto LB and LB + amp, respectively
July 1, 2008
Nathan Puhl
Subcultured RP1616 into liquid LB and pTopp cells into liquid LB + amp
July 2, 2008
Nathan Puhl, Christa, Munima
Plasma mini prepped pTopp cells
Attempted to make RP1616 cells competent using the open wet ware protocol. 100 uL aliquots of cells were froxen in liquid nitrogen and stored in the -80 freezer.
Ran CheZ in pUC19 plasmid and pUC19 plasmid from last year with high range ladder. The pUC19 plasmid seems weird, I don't think it should have two bands; I will have to ask someone about that. The CheZ plasmid appears to be the right size (2600 + 620 = 3220 bp)
