Team:University of Ottawa/3 June 2008

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Today in the lab

Dan, Matt

Yesterday's electrophoresis
  • The electrophoresis indicated that the miniprep did not go as planned, as a result we will have to do it again. Note: We have to number the colonies that we are using for our miniprep because some may not be expressing the plasmid as well as others.
  • We also realized that me might have been using slightly too much DNA in our digestions. We made a calculator to help us determine the right amounts of enzyme, DNA and buffer to use. This should also improve the resolution of our gels.
  • New Miniprep
  • We begann our new miniprep and will make sure to number the colonies. We prepared the miniprep for each plasmid from two different colonies.
  • We left the bacteria to innoculate at 37C overnight at 250 rpm.