Team:University of Sheffield/Rosie

From 2008.igem.org

(Difference between revisions)
(Rosie's Lab Book)
(Biobrick Book Testing)
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After Eva's characterisation failed, we had our suspicions about the BioBrick Registry. However, given our own intermediate skill, and the prescence of many far more likely problems, it wasnt until we started to make the BioBricks that the full scale of the problem was obvious. After extractng and restriction the BioBrick vector pSB10A3, nothing showed up on the gel.
After Eva's characterisation failed, we had our suspicions about the BioBrick Registry. However, given our own intermediate skill, and the prescence of many far more likely problems, it wasnt until we started to make the BioBricks that the full scale of the problem was obvious. After extractng and restriction the BioBrick vector pSB10A3, nothing showed up on the gel.
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[[Image:Gel_7485b.jpg]]
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[[Image:Gel_7485b.jpg|300px|]]
Lane1: Marker
Lane1: Marker
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Below is the gel of 5 extractions from BioBricks all over the registry (emphasis on vectors as thats waht we needed) - our final test to see if the book was faulty or not:
Below is the gel of 5 extractions from BioBricks all over the registry (emphasis on vectors as thats waht we needed) - our final test to see if the book was faulty or not:
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[[Image:Gel_7487_BioBrick_Test.jpg]]
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[[Image:Gel_7487_BioBrick_Test.jpg|400px|]]
Lane 1: Marker
Lane 1: Marker

Revision as of 12:14, 29 October 2008

UniShefBanner.jpg


Introduction Our project Modelling Wet Lab Our team Timetable Miscellaneous


Introduction Protocols

Rosie's Lab Book

Contents


Early Work

Training, involving being familiarised with all the protocols listed with extensive practising


Biobrick Book Testing

After Eva's characterisation failed, we had our suspicions about the BioBrick Registry. However, given our own intermediate skill, and the prescence of many far more likely problems, it wasnt until we started to make the BioBricks that the full scale of the problem was obvious. After extractng and restriction the BioBrick vector pSB10A3, nothing showed up on the gel.

Gel 7485b.jpg

Lane1: Marker

Lanes 2 - 6: BBpSB1AK3 digested


Below is the gel of 5 extractions from BioBricks all over the registry (emphasis on vectors as thats waht we needed) - our final test to see if the book was faulty or not:

Gel 7487 BioBrick Test.jpg

Lane 1: Marker

Lane 2: pSB1AK3

Lane 3: pSB1AC3 source plate:1020 well:4B

Lane 4: pSB1A2 sp:1003 w:4A

Lane 5: pBAD/araC sp:1013 w:5C

Lane 6: 30C6HSL reciever sp:1001 w:12B

Lane 7: Banana odour sp:1021 w:12B


As you can see, there is no DNA present.