Team:Valencia/Notebook/October

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=== October 24th ===
=== October 24th ===
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- Ligating Biobricks into plasmids:  
+
- [[Team:Valencia/Parts#Ligating_Biobricks_into_plasmids|Ligating Biobricks]] into plasmids:  
Inserts were run into agarose gels and DNA bands excised with a clean scalpel. DNA was extracted from agarose blocks (ultra clean gel spin, DNA purification Kit, MO BIO laboratories). T4 Ligase was used to ligate inserts and vectors.
Inserts were run into agarose gels and DNA bands excised with a clean scalpel. DNA was extracted from agarose blocks (ultra clean gel spin, DNA purification Kit, MO BIO laboratories). T4 Ligase was used to ligate inserts and vectors.
-
- Competent cells were transformed and resulting colonies (Amp LB) screened with Fw and Rv primers to confirm the presence of inserts.  
+
- Competent cells were transformed and resulting colonies (Amp LB) screened with Fw and Rv primers to confirm the presence of inserts.
-
=== OCtober 27th ===
+
=== October 27th ===
- pSB1AK3 containing UCP-1, 175-deleted and 76-deleted were sent to the Registry.
- pSB1AK3 containing UCP-1, 175-deleted and 76-deleted were sent to the Registry.

Latest revision as of 22:14, 29 October 2008



April
Mo Tu We Th Fr Sa Su
31  1   2  3  4  5  6
 7  8   9 10 11 12 13
14 15  16 17 18 19 20
21 22  23 24 25 26 27
28 29  30  1  2  3  4
May
Mo Tu We Th Fr Sa Su
28 29  30  1  2  3  4
 5  6   7  8  9 10 11
12 13  14 15 16 17 18
19 20  21 22 23 24 25
26 27  28 29 30 31 1
June
Mo Tu We Th Fr Sa Su
26 27  28  29 30 31  1
 2  3   4  5  6  7  8
 9 10  11 12 13 14 15
16 17  18 19 20 21 22
23 24  25 26 27 28 29
30  1   2  3  4  5  6
July
Mo Tu We Th Fr Sa Su
30  1  2  3  4  5  6
 7  8   9 10 11 12 13
14 15  16 17 18 19 20
21 22  23 24 25 26 27
28 29  30 31   1  2  3
August
Mo Tu We Th Fr Sa Su
28 29  30 31  1  2  3
 4  5   6  7  8  9 10
11 12  13 14 15 16 17
18 19  20 21 22 23 24
25 26  27 28 29 30 31
September
Mo Tu We Th Fr Sa Su
 1  2   3  4  5  6  7
 8  9  10 11 12 13 14
15 16  17 18 19 20 21
22 23  24 25 26 27 28
29 30  1 2 3 4 5
October
Mo Tu We Th Fr Sa Su
29 30    1   2  3  4  5
 6  7   8 9 10 11 12
13 14  15 16 17 18 19
20 21  22 23 24 25 26
27 28  29 30 31  1  2
November
Mo Tu We Th Fr Sa Su
27 28  29 30 31  1  2
 3  4   5  6  7  8  9
10 11  12 13 14 15 16
17 18  19 20 21 22 23
24 25  26 27 28 29 30


October 1st

- We repeated the previous experiment:
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 2
      270 rpm.
       Initial temperature 28 ºC.

October 2nd

- Team meeting: We talked about the biobricks and some experiments left.

October 7th

- We repeated the previous experiment:
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 2
      270 rpm.
       Initial temperature 28 ºC.

October 13th

- Total DNA was extracted from our yeast strains
- UCP-1 was amplified by PCR using oligonucleotides matching the sequence and bearing the appropriate Biobrick prefix and suffix.

October 14th

- We performed an agarose gel electrophoresis in order to check our PCR, the results was: Intense amplicons, of the expected size, (about 1 kb) were produced for UCP-1, 175-deleted and 76 deleted.

October 17th

- Team meeting: We discussed several things that need to be finished, such as sending DNA, preparing presentation, poster, wiki...

October 20th

- Preparing vectors, competent cells were transformed with: pSB1AK3 and pUC18 plasmids.

October 23rd

- Plasmids were extracted (High pure miniprep plasmid isolation kit ROCHE).

- Plasmids were digested with EcoRI and PstI

October 24th

- Ligating Biobricks into plasmids: Inserts were run into agarose gels and DNA bands excised with a clean scalpel. DNA was extracted from agarose blocks (ultra clean gel spin, DNA purification Kit, MO BIO laboratories). T4 Ligase was used to ligate inserts and vectors.

- Competent cells were transformed and resulting colonies (Amp LB) screened with Fw and Rv primers to confirm the presence of inserts.

October 27th

- pSB1AK3 containing UCP-1, 175-deleted and 76-deleted were sent to the Registry.



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