Team:Valencia/Notebook/Protocols
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<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Joadelas/valencia.css&action=raw&ctype=text/css" type="text/css"></html> | <html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Joadelas/valencia.css&action=raw&ctype=text/css" type="text/css"></html> | ||
+ | {{Valencia/Menu}} | ||
<div class="valenciaMain"> | <div class="valenciaMain"> | ||
- | <div style=" | + | <div style=" width:94%; margin: 0 auto;"> |
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+ | == Glycerol Stock == | ||
+ | 2 ml Eppendorf containing: <br> | ||
+ | {| | ||
+ | |Sterile Glycerol (stock 50 %) | ||
+ | |1ml <br> | ||
+ | |- | ||
+ | |Half volume of a culture plate <br> | ||
+ | |} | ||
- | |||
- | |||
== SD Medium == | == SD Medium == | ||
- | Yeast Nitrogen Base 0.67% | + | {| |
- | Glucose | + | |Yeast Nitrogen Base |
- | Agar | + | |0.67% |
- | Casamino acids | + | |- |
- | Tryptophan | + | |Glucose |
- | Adenine | + | |2% |
- | + | |- | |
+ | |Agar | ||
+ | |2% | ||
+ | |- | ||
+ | |Casamino acids | ||
+ | |0.1% | ||
+ | |- | ||
+ | |Tryptophan | ||
+ | |20 mg/l | ||
+ | |- | ||
+ | |Adenine | ||
+ | |40 mg/l | ||
+ | |} | ||
== SP liquid medium == | == SP liquid medium == | ||
- | Yeast Nitrogen Base 0.67% | + | {| |
- | Potassium phosohate 0.1% | + | |Yeast Nitrogen Base |
- | Ammonium sulfate 0.12% | + | |0.67% |
- | Glucose 0.05% | + | |- |
- | Lactic acid 2% | + | |Potassium phosohate |
- | Casamino acids 0.1% | + | |0.1% |
- | Tryptophan 20 mg/l | + | |- |
- | Adenine 40 mg/l | + | |Ammonium sulfate |
- | + | |0.12% | |
+ | |- | ||
+ | |Glucose | ||
+ | |0.05% | ||
+ | |- | ||
+ | |Lactic acid | ||
+ | |2% | ||
+ | |- | ||
+ | |Casamino acids | ||
+ | |0.1% | ||
+ | |- | ||
+ | |Tryptophan | ||
+ | |20 mg/l | ||
+ | |- | ||
+ | |Adenine | ||
+ | |40 mg/l | ||
+ | |} | ||
- | == YPKAc Medium | + | == YPKAc Medium == |
- | Yeast extract 1% | + | {| |
- | Peptone 2% | + | |Yeast extract |
- | Potassium acetate | + | |1% |
- | Tryptophan 20 mg/l | + | |- |
- | Adenine 40 mg/l | + | |Peptone |
+ | |2% | ||
+ | |- | ||
+ | |Potassium acetate | ||
+ | |2% | ||
+ | |- | ||
+ | |Tryptophan | ||
+ | |20 mg/l | ||
+ | |- | ||
+ | |Adenine | ||
+ | |40 mg/l | ||
+ | |} | ||
</div> | </div> |
Latest revision as of 12:55, 5 October 2008
Glycerol Stock
2 ml Eppendorf containing:
Sterile Glycerol (stock 50 %) | 1ml |
Half volume of a culture plate |
SD Medium
Yeast Nitrogen Base | 0.67% |
Glucose | 2% |
Agar | 2% |
Casamino acids | 0.1% |
Tryptophan | 20 mg/l |
Adenine | 40 mg/l |
SP liquid medium
Yeast Nitrogen Base | 0.67% |
Potassium phosohate | 0.1% |
Ammonium sulfate | 0.12% |
Glucose | 0.05% |
Lactic acid | 2% |
Casamino acids | 0.1% |
Tryptophan | 20 mg/l |
Adenine | 40 mg/l |
YPKAc Medium
Yeast extract | 1% |
Peptone | 2% |
Potassium acetate | 2% |
Tryptophan | 20 mg/l |
Adenine | 40 mg/l |