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Team:Valencia/Notebook/Protocols
From 2008.igem.org
(Difference between revisions)
| Line 39: | Line 39: | ||
== SP liquid medium == | == SP liquid medium == | ||
| - | Yeast Nitrogen Base 0.67% | + | {| |
| - | Potassium phosohate 0.1% | + | |Yeast Nitrogen Base |
| - | Ammonium sulfate 0.12% | + | |0.67% |
| - | Glucose 0.05% | + | |- |
| - | Lactic acid 2% | + | |Potassium phosohate |
| - | Casamino acids 0.1% | + | |0.1% |
| - | Tryptophan 20 mg/l | + | |- |
| - | Adenine 40 mg/l | + | |Ammonium sulfate |
| - | + | |0.12% | |
| + | |- | ||
| + | |Glucose | ||
| + | |0.05% | ||
| + | |- | ||
| + | |Lactic acid | ||
| + | |2% | ||
| + | |- | ||
| + | |Casamino acids | ||
| + | |0.1% | ||
| + | |- | ||
| + | |Tryptophan | ||
| + | |20 mg/l | ||
| + | |- | ||
| + | |Adenine | ||
| + | |40 mg/l | ||
| + | |} | ||
== YPKAc Medium == | == YPKAc Medium == | ||
| - | Yeast extract 1% | + | {| |
| - | Peptone 2% | + | |Yeast extract |
| - | Potassium acetate | + | |1% |
| - | Tryptophan 20 mg/l | + | |- |
| - | Adenine 40 mg/l | + | |Peptone |
| + | |2% | ||
| + | |- | ||
| + | |Potassium acetate | ||
| + | |2% | ||
| + | |- | ||
| + | |Tryptophan | ||
| + | |20 mg/l | ||
| + | |- | ||
| + | |Adenine | ||
| + | |40 mg/l | ||
| + | |} | ||
</div> | </div> | ||
Latest revision as of 12:55, 5 October 2008
Glycerol Stock
2 ml Eppendorf containing:
| Sterile Glycerol (stock 50 %) | 1ml |
| Half volume of a culture plate |
SD Medium
| Yeast Nitrogen Base | 0.67% |
| Glucose | 2% |
| Agar | 2% |
| Casamino acids | 0.1% |
| Tryptophan | 20 mg/l |
| Adenine | 40 mg/l |
SP liquid medium
| Yeast Nitrogen Base | 0.67% |
| Potassium phosohate | 0.1% |
| Ammonium sulfate | 0.12% |
| Glucose | 0.05% |
| Lactic acid | 2% |
| Casamino acids | 0.1% |
| Tryptophan | 20 mg/l |
| Adenine | 40 mg/l |
YPKAc Medium
| Yeast extract | 1% |
| Peptone | 2% |
| Potassium acetate | 2% |
| Tryptophan | 20 mg/l |
| Adenine | 40 mg/l |
