Team:Valencia/Notebook/Protocols

From 2008.igem.org

Revision as of 19:08, 18 August 2008 by Joadelas (Talk | contribs)

Home   |  The Team   |  The Project   |  Parts Submitted to the Registry   |  Modeling   |  Notebook


Glycerol Stock

2 ml Eppendorf containing:
Sterile Glycerol (stock 50 %) 1ml
Half culture plate


SD Medium

Yeast Nitrogen Base 0.67%
Glucose 2%
Agar 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l


SP liquid medium

Yeast Nitrogen Base 0.67%
Potassium phosohate 0.1%
Ammonium sulfate 0.12%
Glucose 0.05%
Lactic acid 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l


YPKAc Medium

Yeast extract 1%
Peptone 2%
Potassium acetate 2%
Tryptophan 20 mg/l
Adenine 40 mg/l