Team:Valencia/Notebook/Protocols
From 2008.igem.org
Glycerol Stock
2 ml Eppendorf containing:
Sterile Glycerol (stock 50 %) 1ml
Half culture plate
SD Medium
Yeast Nitrogen Base 0.67%
Glucose 2%
Agar 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l
SP liquid medium
Yeast Nitrogen Base 0.67%
Potassium phosohate 0.1%
Ammonium sulfate 0.12%
Glucose 0.05%
Lactic acid 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l
YPKAc Medium
Yeast extract 1%
Peptone 2%
Potassium acetate 2%
Tryptophan 20 mg/l
Adenine 40 mg/l