Team:Valencia/Notebook/Protocols

From 2008.igem.org


Glycerol Stock

2 ml Eppendorf containing:

Sterile Glycerol (stock 50 %) 1ml
Half volume of a culture plate


SD Medium

Yeast Nitrogen Base   0.67%
Glucose 2%
Agar 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l

SP liquid medium

Yeast Nitrogen Base 0.67%
Potassium phosohate   0.1%
Ammonium sulfate 0.12%
Glucose 0.05%
Lactic acid 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l

YPKAc Medium

Yeast extract 1%
Peptone 2%
Potassium acetate   2%
Tryptophan 20 mg/l
Adenine 40 mg/l