Team:Virginia

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<a href="https://2008.igem.org/Team:Virginia/Project"><img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/icons/project.gif">Projects&nbsp;</a><span onClick="showHide('projects')" class=expander>&raquo;</span><br>
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<a href="#">Transcription Attenuators</a><br>
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<a href="#">Placeholder Sites</a><br>
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<a href="#">BioPlastic</a><br>
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<a href="#">Adding to the RSBP</a><br>
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<a href="https://2008.igem.org/Team:Virginia/Parts"><img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/icons/parts.gif">BioBricks</a><br>
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<a href="https://2008.igem.org/Team:Virginia/Results"><img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/icons/modeling.gif">Results</a><br>
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<a href="https://2008.igem.org/Team:Virginia/Notebook"><img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/icons/notebook.gif">Notebook</a><br>
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<a href="http://www4.clustrmaps.com/counter/maps.php?url=https://2008.igem.org/Team:Virginia" id="clustrMapsLink"><img class="logos" src="http://www4.clustrmaps.com/counter/index2.php?url=https://2008.igem.org/Team:Virginia" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';" /></a>
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<br><span>We'd like to thank our generous sponsors for making our work possible:</span><br>
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<a href="http://www.virginia.edu/"><img class="logos" src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/uva-logo-patch.png" alt="University of Virginia" title="University of Virginia" /></a>
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<a href="http://dupont.com"><img class="logos" src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/dupont.gif" alt="duPont" title="duPont" /></a>
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<h2>VGEM 2008: Providing you with the tools for 2009</h2>
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<img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/board.jpg" width=650>
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<h3></h3>
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<p>A main challenge in constructing synthetic biological systems is the inability to precisely regulate gene expression using artificial means. Tightly-regulated control of any given set of related transcriptional, translational and posttranslational events will likely require a combination of powerful strategies. Therefore, the 2008 Virginia iGEM team is developing a library of transcriptional terminators intentionally redesigned to be functionally inefficient. Well-characterized, standardized terminators of various efficiencies should allow finely-tuned transcription attenuation and represents yet another step toward global biological control. This work complements other gene expression control methods that focus on initiation of transcription. The desired result is quantitative control of transcript levels, which is often necessary to balance flux through a synthetic metabolic pathway. To demonstrate its potential for real-world application, the team is planning to employ this approach to control the expression of a heterologous pathway in E. coli for the biosynthesis of polyhydroxybutyrate (PHB), a biodegradable polyester plastic.</p>
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<h2>2008 VGEM Team</h2>
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<h3>Genetic Attenuators</h3>
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<p>Welcome to the Virginia Genetically Engineered Machine Team's official iGEM wiki! The VGEM team is in its
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<p>Getting in control of transcription</p>
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second year of competition and is looking forward to participating in the 2008 iGEM Jamboree.<p>
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<p>Terminators are never 100% efficient, meaning that not all polymerases will disengage from the strand they are operating on. This phenomenon can be exploited to create <i>Genetic Attentuators</i>. Inserting a <i>Genetic Attenuator</i> between genes will create transcripts containing different sets of genes. Varying the number of copies of a gene present in the transcript will influence downstream translation of that gene.
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</p>
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<center><img src="https://static.igem.org/mediawiki/2008/thumb/b/b8/Team.jpg/800px-Team.jpg"  alt="Thea VGEM Team" width="500"/></center>
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<span>MORE</span>
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<h2>Research Overview</h2>
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Synthetic biological systems are useful both to empirically study fundamental biological behavior and to solve applied biological problems.  One of the main challenges in constructing synthetic biological systems is the inability to precisely regulate gene expression using artificial means. It is likely that only a combination of powerful strategies will result in tightly-regulated control of any given set of related transcriptional, translational and posttranslational events, which is crucial to advancing the field of synthetic biology. Therefore, the 2008 VGEM Team is developing a new tool for biological engineering, transcriptional terminators intentionally redesigned to be functionally inefficient. A well-characterized library of standardized transcriptional terminators of various efficiencies should allow finely-tuned transcriptional attenuation by controlling termination efficiency and represents yet another step toward global biological control. This work represents a complimentary approach to other gene expression control methods that focus on initiation of transcription.  The desired result is precise, quantitative control of transcript levels, which is often necessary to balance flux through a synthetic metabolic pathway. To demonstrate its potential for real-world application, the team is planning to employ this approach to control the expression of a heterologous pathway in E. coli for the synthesis of polyhydroxybutyrate (PHB), a biodegradable polyester plastic derived from renewable biomass, not from petroleum sources.
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<h3>Placeholder Sites</h3>
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<p>Cloning sites made easy!</p>
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<p>Assembling gene constructs is a tedious procedure that consumes valuable time. When assembling several BioBricks into a vector it would be valuable to be able to insert a cloning site which could later be used to insert a BioBrick into a certain part of the vector. <i>Placeholder Sites</i> accomplish this task by providing restriction sites compatible with the BioBrick standard inside of a standard part.</p>
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<ol>
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<li>EX - ApoI (NotI) AvrII - SP</li>
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<li>EX - ApoI (NotI) NheI - SP</li>
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<li>EX - ApoI (NotI) NsiI - SP</li>
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<li>EX - ApoI (NotI) SbfI - SP</li>
 +
<li>EX - MfeI (NotI) AvrII - SP</li>
 +
<li>EX - MfeI (NotI) NheI - SP</li>
 +
<li>EX - MfeI (NotI) Nsil - SP</li>
 +
<li>EX - MfeI (NotI) SbfI - SP</li>
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</ol>
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<span>MORE</span>
</div>
</div>
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<div class="box">
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<h3>BioPlastic</h3>
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<p>Growing a renewable resource</p>
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<p>Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.</p>
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<img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/pathway-small.jpg" />
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<p>et cetera</p>
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<span>MORE</span>
</div>
</div>
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<div class="box">
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<h3>Additions to the Registry</h3>
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<p>Giving back</p>
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<p>Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.</p>
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<span>MORE</span>
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Revision as of 01:40, 30 October 2008


Home
People
Projects »
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Results
Notebook
Locations of visitors to this page
We'd like to thank our generous sponsors for making our work possible:
University of Virginia duPont

VGEM 2008: Providing you with the tools for 2009

A main challenge in constructing synthetic biological systems is the inability to precisely regulate gene expression using artificial means. Tightly-regulated control of any given set of related transcriptional, translational and posttranslational events will likely require a combination of powerful strategies. Therefore, the 2008 Virginia iGEM team is developing a library of transcriptional terminators intentionally redesigned to be functionally inefficient. Well-characterized, standardized terminators of various efficiencies should allow finely-tuned transcription attenuation and represents yet another step toward global biological control. This work complements other gene expression control methods that focus on initiation of transcription. The desired result is quantitative control of transcript levels, which is often necessary to balance flux through a synthetic metabolic pathway. To demonstrate its potential for real-world application, the team is planning to employ this approach to control the expression of a heterologous pathway in E. coli for the biosynthesis of polyhydroxybutyrate (PHB), a biodegradable polyester plastic.

Genetic Attenuators

Getting in control of transcription

Terminators are never 100% efficient, meaning that not all polymerases will disengage from the strand they are operating on. This phenomenon can be exploited to create Genetic Attentuators. Inserting a Genetic Attenuator between genes will create transcripts containing different sets of genes. Varying the number of copies of a gene present in the transcript will influence downstream translation of that gene.

MORE

Placeholder Sites

Cloning sites made easy!

Assembling gene constructs is a tedious procedure that consumes valuable time. When assembling several BioBricks into a vector it would be valuable to be able to insert a cloning site which could later be used to insert a BioBrick into a certain part of the vector. Placeholder Sites accomplish this task by providing restriction sites compatible with the BioBrick standard inside of a standard part.

  1. EX - ApoI (NotI) AvrII - SP
  2. EX - ApoI (NotI) NheI - SP
  3. EX - ApoI (NotI) NsiI - SP
  4. EX - ApoI (NotI) SbfI - SP
  5. EX - MfeI (NotI) AvrII - SP
  6. EX - MfeI (NotI) NheI - SP
  7. EX - MfeI (NotI) Nsil - SP
  8. EX - MfeI (NotI) SbfI - SP
MORE

BioPlastic

Growing a renewable resource

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.

et cetera

MORE

Additions to the Registry

Giving back

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.

MORE

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