Team:Warsaw

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<p>We have developed a system allowing to search for antibodies with new specificities or screen protein libraries in order to generate interactors for a given bait. Our system changes a protein sequence to maximize its interaction with a given partner. Proteins with modified sequences are then directed to the bacterial outer membrane, where the best interactors are selected. Protein presented on the cell surface is fused with part of &#946;-lactamase protein, while its bait is fused with the complementing part of the enzyme and added to medium. The stronger the interaction between proteins of interest, the more efficient the binding of the two halves of &#946;-lactamase, leading to resistance to ampicillin and survival. Cells expressing less interacting variants die as they don't achieve sufficient complementation of the reporter enzyme. This allows us to select strains producing interactors for any given bait. </p>
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<h1>A novatory system for generation and screening for new antibody specificities against any bait</h1>
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<li>maximization of antibody-given bait interaction </li><li>
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interactor presentation on bacterial surface</li><li>
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selection of best bait interactor</li>
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     <a href="http://www.fundacjauv.org.pl/"><h3>The Universitatis Varsoviensis Fund </h3></a></td>
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     <a href="http://www.fundacjauv.org.pl/"><h3>The Universitatis Varsoviensis Foundation </h3></a></td>
     <td align="center" valign="bottom"><a href="http://www.fnp.org.pl/ang/index.html"><img src="https://static.igem.org/mediawiki/2008/c/c9/LogoFNP1wers.gif" width="221" height="75" border="0" /></a>
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     <a href="http://www.fnp.org.pl/ang/index.html"><h3>Foundation for Polish Science </h3></a></td>
     <a href="http://www.fnp.org.pl/ang/index.html"><h3>Foundation for Polish Science </h3></a></td>
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<a href="http://www.iimcb.gov.pl/zylicz_lab.php"><h3>Department of Molecular Biology, IIMCB</h3></a>
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<a href="http://www.iimcb.gov.pl/zylicz_lab.php"><h3>Department of Molecular Biology, International Institute of Molecular and Cell Biology</h3></a>
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<p>Departments belonging to the <a href="http://www.biol.uw.edu.pl/_2008eng/">Faculty of Biology, University of Warsaw</a>, who kindly provided access to laboratory space and equipment:</p>
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<p>Units belonging to the <a href="http://www.biol.uw.edu.pl/_2008eng/">Faculty of Biology, University of Warsaw</a>, who kindly provided access to laboratory space and equipment:</p>
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Revision as of 17:54, 29 October 2008

Gallery Bricks Notebook Team Project Home

A novatory system for generation and screening for new antibody specificities against any bait

  • maximization of antibody-given bait interaction
  • interactor presentation on bacterial surface
  • selection of best bait interactor

Institutions whom we would like to thank for financial and technical support:

University of Warsaw

Faculty of Biology, University of Warsaw

The Universitatis Varsoviensis Foundation

Foundation for Polish Science

Warsaw University Foundation

Department of Molecular Biology, International Institute of Molecular and Cell Biology

Units belonging to the Faculty of Biology, University of Warsaw, who kindly provided access to laboratory space and equipment:

Department of Plant Molecular Biology

Department of Virology

Department of Bacterial Genetics

Institute of Genetics and Biotechnology

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