Team:Warsaw/Calendar-Main/11 July 2008

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<p>'''Preparation of construct A-omega'''<br>
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1. PCR  <br>
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Revision as of 15:38, 26 September 2008

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Preparation of constructs with OmpA protein fusions
1. Colony PCR on colonies from plates with transformations OmpA_alpha.
2. Confirmed transformant colonies inoculated to liquid LB with kanamycin.
Cloning of protein Z DNA to OmpA constructs
1. 2 colonies was inoculated to liquid LB broth with kanamycin

Preparation of construct A-omega
1. PCR