Team:Warsaw/Calendar-Main/11 July 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

  1. Colony PCR on colonies from plates with transformations OmpA_alpha.
  2. Gel electrophoresis.
  3. Confirmed transformant colonies inoculated to liquid LB with kanamycin.

Preparation of construct pKS with A protein

Michał L., Ewa, Marcin

  1. Colony PCR on colonies from plates with transformations pKS-A.
    Primers used: AL+SacI and AP+NotI
  2. Gel electrophoresis.
  3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.