Team:Warsaw/Calendar-Main/12 July 2008

From 2008.igem.org

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<h3>Preparation of constructs with OmpA protein fusions</h3> <h4>Michał K.</h4>
<h3>Preparation of constructs with OmpA protein fusions</h3> <h4>Michał K.</h4>
<p><ol>
<p><ol>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (pCACYC177 + OmpA_alpha). </li>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pCACYC177 + OmpA_alpha</a>). </li>
<li> Control  
<li> Control  
<a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li> <li> Gel electrophoresis - we found good clones.</li>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li> <li> Gel electrophoresis - we found good clones.</li>
</ol></p>
</ol></p>
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<h3>Preparation of construct pKS with A protein</h3> <h4>Michał L., Ewa, Marcin</h4>
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<h3>Preparation of construct <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII+A>pKS with A protein</a></h3> <h4>Michał L., Ewa, Marcin</h4>
<p><ol>
<p><ol>
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmid DNA</a> from cultures inocluated on previous day. </li>
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmid DNA</a> from cultures inocluated on previous day. </li>
<li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmid with SacI and NotI (BamHI buffer) - 2 h.</li>
<li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmid with SacI and NotI (BamHI buffer) - 2 h.</li>
<li> Gel electrophoresis of digested DNA.</li>
<li> Gel electrophoresis of digested DNA.</li>
-
<li> We are glad to find the proper clone and inoculate liquid LB with ampicillin to freeze bacteria carrying pKS-A construct.</li></ol>
+
<li> We are glad to find the proper clone and inoculate liquid LB with ampicillin to freeze bacteria carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII+A>pKS-A</a> construct.</li></ol>
</p>
</p>

Revision as of 11:56, 13 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pCACYC177 + OmpA_alpha).
  2. Control digest of isolated plasmids with BamHI and SacI (BamHI buffer).
  3. Gel electrophoresis - we found good clones.

Preparation of construct pKS with A protein

Michał L., Ewa, Marcin

  1. Isolation of plasmid DNA from cultures inocluated on previous day.
  2. Control digest of isolated plasmid with SacI and NotI (BamHI buffer) - 2 h.
  3. Gel electrophoresis of digested DNA.
  4. We are glad to find the proper clone and inoculate liquid LB with ampicillin to freeze bacteria carrying pKS-A construct.

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