Team:Warsaw/Calendar-Main/12 July 2008
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmid DNA</a> from cultures inocluated on previous day. </li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmid DNA</a> from cultures inocluated on previous day. </li> | ||
<li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmid with SacI and NotI (BamHI buffer) - 2 h.</li> | <li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmid with SacI and NotI (BamHI buffer) - 2 h.</li> | ||
- | <li> Gel electrophoresis of digested DNA | + | <li> Gel electrophoresis of digested DNA.</li> |
<li> We are glad to find the proper clone and inoculate liquid LB with ampicillin to freeze bacteria carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII+A>pKS-A</a> construct.</li></ol> | <li> We are glad to find the proper clone and inoculate liquid LB with ampicillin to freeze bacteria carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII+A>pKS-A</a> construct.</li></ol> | ||
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pCACYC177 + OmpA_alpha</a>). </li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pCACYC177 + OmpA_alpha</a>). </li> | ||
<li> Control | <li> Control | ||
- | <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li> <li> Gel electrophoresis - we found good clones.</li> | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li> <li> Gel electrophoresis - we found good clones (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/12_July_2008#fig1">Fig. 1.</a>).</li> |
</ol></p> | </ol></p> | ||
- | <img src="https://static.igem.org/mediawiki/2008/2/28/Cztery.jpg" width=300 /><var><b>Fig. 1. Control SacI/BamHI digests of plasmids which had a colony PCR product | + | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/2/28/Cztery.jpg" width=300 /></a><var><b>Fig. 1. </b>Control SacI/BamHI digests of plasmids which had a colony PCR product<br> |
1. Marker<br> | 1. Marker<br> | ||
2-4. digested plasmids which had a colony PCR product<br></var> | 2-4. digested plasmids which had a colony PCR product<br></var> |
Latest revision as of 17:46, 26 October 2008
Preparation of construct pKS with A proteinMichał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpAPiotr, Antoni
Preparation of constructs: OmpA_alpha and OmpA_omega #2Michał K.
1. Marker 2-4. digested plasmids which had a colony PCR product |