Team:Warsaw/Calendar-Main/12 May 2008

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<html><h3>Preparation of pMPMT5+AID construct</h3>
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<h4>Michał K.:</h4>
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Michał K.</h3>
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<li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#electrotransform">electroporation</a> with ligation (pMPM-T5+AID).</li>
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1. E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#electrotransform">electroporation</a> with ligation (pMPM-T5+AID).</p>
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<li>Plating on LB + tetracycline.</li></ol></p>
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<p>2. Plating on LB + tetracycline.</p>
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Revision as of 13:51, 9 October 2008

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Preparation of pMPMT5+AID construct
Michał K.

  1. E. coli TOP10 electroporation with ligation (pMPM-T5+AID).
  2. Plating on LB + tetracycline.

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