Team:Warsaw/Calendar-Main/14 July 2008

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<p>'''Cloning of protein Z DNA to OmpA constructs'''<br>
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<h3>Cloning of protein Z DNA to OmpA constructs</h3>
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1. Digestion and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI. <br> </p>
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<p><ol>
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2. Gel eloctrophoresis and gel-out (4300 bp band). <br>
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<li> Digestion and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI. </li>
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3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July. <br>
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<li> Gel eloctrophoresis and gel-out (4300 bp band). </li>
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4. Transformation of E. coli TOP10 strain with ligation. <br>
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<li> Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July. </li>
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5. Transformants plating on LB + kanamycin.  
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<li> Transformation of E. coli TOP10 strain with ligation. </li>
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<li> Transformants plating on LB + kanamycin. </li>
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</ol></p>
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Cloning of protein Z DNA to OmpA constructs

  1. Digestion and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI.
  2. Gel eloctrophoresis and gel-out (4300 bp band).
  3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July.
  4. Transformation of E. coli TOP10 strain with ligation.
  5. Transformants plating on LB + kanamycin.


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