Team:Warsaw/Calendar-Main/18 September 2008
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<li> Gel electrophoresis of digested plasmid | <li> Gel electrophoresis of digested plasmid | ||
- | and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band (OmpA_linker - 500 bp) | + | and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band (OmpA_linker - 500 bp). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/18_September_2008#fig5">Fig. 5.</a>.</li> |
<p class="hide"> | <p class="hide"> | ||
<img src="https://static.igem.org/mediawiki/2008/6/6a/Go_29_09.jpg" width=300/><var><b>Fig. 5.</b> SacI/NdeI digest of pET15b_OmpA_omega <br> | <img src="https://static.igem.org/mediawiki/2008/6/6a/Go_29_09.jpg" width=300/><var><b>Fig. 5.</b> SacI/NdeI digest of pET15b_OmpA_omega <br> |
Revision as of 13:35, 27 October 2008
'Hunter and prey' system tests: Competition testsWeronikaInoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_Z_omega, pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_alpha + induction using 0.25mM iPTG MutD5 testingPiotrInoculation of mutD5 into 100 ml of LB and rendering them chemocompetent. Electroporation with pACYC177+OmpA_Z_alpha, pACYC177+OmpA_Z_omega, pACYC177+OmpA_A_omega and pACYC177+OmpA_omega_ΔA. Optimisation of primers for preparation of partsMichał K.
1. Marker 2. 55 °C linker_alpha 3. 60 °C linker_alpha 4. 65 °C linker_alpha 5. 70 °C linker_alpha Preparation of ΔA (BBa_K103003)Michał K.
Preparation of linker_alpha (BBa_K103009)Michał K.
Preparation of linker_omega (BBa_K103013)Michał K.
Preparation of OmpA-linker-omega-linker (BBa_K103016)Michał K.
Preparation of OmpA-linker (BBa_K103006)Michał K.
Fig. 5. SacI/NdeI digest of pET15b_OmpA_omega 1. Marker 2. deltaA 3. omega 4. ompA_omega Fig. 3. EcoRI/BcuI digest of pSB1A3 1. Marker 2. Digested pSB1A3 Fig. 4. PCR to obtain linker_alpha 1. Marker 2. linker_alpha Fig. 5. SacI/NdeI digest of pET15b_OmpA_omega 1. Marker 2. digested pSB1A3
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