Michał K.:

1. Gel electrophoresis (16 May PCR's) and DNA isolation from proper bands (600 bp for AID lane and 2700 bp for T7 polymerase lanes).

Michał L., Ewa, Marcin.

Overnight inoculation and induction:

Top10 + 0,5 mM IPTG

pMPMAID + Glc + 0,01% L-Ara

pTRCAID + Glc + 0,5 mM IPTG